Polyamide Membrane Circle (NL16), 0.2um Pore Size, 50mm, 100/Pk SKU: 10414014

Nylon Membrane Filters - 10414014

Engineered for high retention efficiency and superior chemical stability, the Whatman 10414014 achieves reliable separation results across a broad range of analytical and preparative applications. Precision membrane geometry balances retention sharpness with sustainable flow rates, minimizing the pressure buildup that leads to premature filter replacement. Working in concert, precision manufacturing ensures that each membrane disc delivers the rated retention, flow rate, and surface consistency that analytical and microbiological methods require.

Material Specifications

• Flexible and durable, eliminating the need for wetting agents that could contaminate samples.
• Ideal for filtering aqueous biological solutions and mobile phases.
• Alkaline-resistant and compatible with a broad range of aqueous buffers used in pharmaceutical and biotechnology laboratories.
• Economical bulk packaging supports laboratories with high consumable throughput where individual sterile wrapping is unnecessary.
• Naturally hydrophilic and constructed with high tensile strength, Nylon provides reliable performance without pre-wetting in aqueous applications.

Scaling your method from development to routine production requires membrane consistency at every lot — the documented specifications of these membranes provide the reproducibility needed to validate the filtration step once. For this application, the natural wettability of nylon allows you to begin filtration immediately upon sample contact, eliminating the bubble formation and flow interruption that hydrophobic membranes introduce when processing aqueous samples without pre-treatment. Make the decision for consistency and reliability, ensuring that your laboratory maintains its reputation for high-quality, error-free output.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

50-65psi

Color

white

Diameter

50mm

Extractables

low

Filtration Area

19.63cm²

Flow Rate

20ml (Water)

Grid (Yes/No)

No

Material

Nylon

Maximum Operating Pressure

Holder-dependent

Maximum Operating Temperature (°C)

135°C

Membrane Thickness (µm)

110um

Pack Count

100

pH Range

3-14

Pore Size (µm)

0.2

Sterility

Nonsterile

Sterilization

Autoclavable at 121°C for 30 mins

Support Material

None

Wettability

Hydrophilic

• Radiolabeled Assays - Retains radiolabeled DNA/RNA fragments efficiently, ensuring accurate quantification in molecular biology experiments.
• Alkaline Filtration - Withstands high-pH solutions during industrial fluid processing, offering durability where other polymers might degrade.
• Atmospheric Testing - Used in dry deposition sampling to capture specific atmospheric nitrates and sulfates for environmental quality studies.
• Solvent Mobilization - Clarifies organic mobile phases in glass vacuum holders, preventing particulate damage to sensitive HPLC pump seals.
• Gene Mapping Support - Provides a stable, charge-modified surface for DNA immobilization, facilitating precise gene mapping and analysis steps.
• Solid Phase Extraction - Acts as a frit or barrier in custom extraction columns, retaining sorbent beds while allowing solvent to pass through.