Sealable Hybridization Reaction Folders, 203 x 254mm, Heat-Sealable, Nonsterile, 50/Pk
Whatman SKU: 10483064

Featuring a polyester and polyethylene laminated film construction, these sealable reaction folders create a clean, enclosed environment for hybridization and incubation reactions in molecular biology workflows. The 203 x 254mm folders are open on three sides, allowing easier insertion of a wet blotting membrane compared to standard hybridization bags with narrow openings. Each folder seals with a standard laboratory heat-sealer to maintain a contamination-free chamber throughout incubation, and the pack provides 50 single-use folders.

Key Construction Characteristics

• Three-sided open design provides unobstructed access for positioning delicate wet membranes without folding or creasing
• Polyester and polyethylene laminated film delivers seal integrity and durability for incubation reactions
• Heat-sealable with standard laboratory sealing equipment for a secure, contamination-free enclosure
• 203 x 254mm (8 x 10 inch) format accommodates standard blot membranes
• Nonsterile, single-use design supplied 50 per pack to reduce cross-contamination between runs

Membrane Compatibility

• Compatible with nitrocellulose membranes for DNA and protein detection protocols
• Compatible with nylon membranes for nucleic acid hybridization
• Compatible with PVDF membranes for protein immunodetection

The three-sided access design reduces the membrane damage risk and setup time that narrow-opening bags create during multi-step hybridization workflows. Sealing the folder maintains a clean, enclosed reaction environment through the full incubation period, helping preserve sample integrity and probe performance.

Color

Transparent

Construction

Three-sided open design

Dimensions

203mm x 254mm

Incompatibility

Compatible with Nitrocellulose, Nylon, and PVDF membranes

Material

Polyester and polyethylene laminate

Pack Count

50

Qualitative/ Quantitative

Qualitative

Sterility

Nonsterile

Sterilization

Do not sterilize: Single-use plastic

• Southern Blotting Provides a contamination-free environment for DNA probe hybridization on nitrocellulose or nylon membranes during genomic analysis.
• Northern Blotting Creates a sealed incubation chamber for RNA hybridization procedures in gene expression and transcript analysis workflows.
• Western Blotting Maintains clean antibody incubation conditions during immunodetection of proteins transferred to PVDF or nitrocellulose membranes.
• Dot Blot Hybridization Encloses spotted membranes during probe binding steps for rapid nucleic acid or protein detection screening procedures.
• Membrane Reprobing Isolates membranes during stripping and re-hybridization cycles that extend data collection from a single blot preparation.
• Colony Lift Screening Protects bacterial or phage colony lifts during probe hybridization steps in recombinant clone identification protocols.