Anotop 25mm Sterile Syringe Filters, 0.1um, 50 Pk
Whatman SKU: 6809-2112

Whatman Anotop Aluminum Oxide Syringe Filters, 0.1 µm, 25 mm, 50/Pk - 6809-2112

Whatman's Anotop line separates by physical particle dimension alone, a capability rooted in ceramic media chemistry rather than the organic polymer chemistries used in most other membrane technologies. Sterilizing-grade syringe filters deliver sterile-grade retention as a single-use format, eliminating the autoclaving and cleaning cycle a reusable sterile filtration setup would require. The alumina membrane tolerates high temperatures and broad solvent ranges and meets dissolved proteins with mineral oxide chemistry throughout. Polypropylene construction resists chemical degradation from routine sample matrices while pressing snugly against the membrane during the pass and sheds virtually no extractable material into the sample. Where bacterial contamination must stay out of the sample, the sub-micron pore rating clears it before the sample reaches the instrument.

Technical Specifications

• Branched-pore alumina structure yields uniform unilamellar liposomes in fewer extrusion passes than polycarbonate membranes
• Minimal extractable content supports HPLC mobile phase filtration without interfering with downstream detection
• Naturally hydrophilic ceramic surface wets without pre-wetting in aqueous samples
• Absolute pore-size rating gives consistent retention across protocols
• Low intrinsic autofluorescence limits background signal in epifluorescence staining with acridine orange and DAPI

Housing Specifications

• Minimal ionic background from the polypropylene housing supports ion chromatography and trace-metal ICP-MS sample preparation.
• Plasticizer-free polyolefin construction keeps the fluid path clean
• Minimal intrinsic autofluorescence from the polypropylene housing supports background-sensitive fluorescence-based assay work downstream.
• Polypropylene housing retains its chemical integrity under vaporized hydrogen peroxide decontamination cycles used in aseptic processing isolators.

A clean particulate separation keeps your aqueous sample preparation dependable. Consistent aqueous clarification clears particulates without slowing your bench routine the same way every time you run it. Ask how Whatman's track record as a proven brand supports standardizing your filtration workflow.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

6.2 bar (90 psi)

Burst Pressure (psi)

100psi

Connection Type

Luer-Lok/ Luer Slip

Construction

Anopore (Aluminum Oxide)

Diameter

25mm

Filtration Area

4.15cm²

Holdup Volume

<150µl

Material

Anopore

Maximum Operating Pressure

100psi

Maximum Operating Temperature (°C)

40°C

Pack Count

50

Pore Size (µm)

0.1

Prefilter (Yes/No)

No

Sterility

Nonsterile

Volume Throughput (ml)

Up to 100ml

Wettability

Hydrophilic

• Low Background Specs - Prepare samples for spectrophotometry, ensuring extremely low optical background for the most sensitive analytical readings.
• Liposome Extrusion - Size liposomes into uniform vesicles precisely, enabling consistent drug delivery research with defined pore structures.
• HPLC Solvent Filtration - Filter chromatography mobile phases and solvents reliably, resisting degradation from aggressive organic reagents.
• Epifluorescence Counting - Counts bacteria and viruses precisely in water samples, using the low-autofluorescence surface for microscopy.
• Gravimetric Solids Testing - Determine particulate mass accurately, using a non-hygroscopic ceramic that resists moisture-driven drift.
• Mycoplasma Removal - Filter tissue culture media reliably, ensuring freedom from Mycoplasma contamination with precise 0.1 µm pore retention.