Anotop 25mm Sterile Syringe Filters, 0.1um, 50 Pk
Whatman SKU: 6809-2112

Whatman Anotop Aluminum Oxide Syringe Filters, 0.1 µm, 25 mm, 50/Pk - 6809-2112

Whatman's Anotop line separates by physical particle dimension alone, a capability rooted in ceramic media chemistry rather than the organic polymer chemistries used in most other membrane technologies. A sterilizing-grade syringe filter carries manufacturer bacterial retention testing built into the design, giving analysts documented sterile-grade performance without repeating that testing in-house for the format. The alumina membrane achieves bacterial retention at the rated pore size while pulling liquid the membrane's full depth at once and stays compatible with aqueous samples, alcohols, and organic solvents. Thermal-bonded PP construction leaves no seam for sample to bypass the membrane while providing a factory-sterilized fluid path suited to aseptic sample preparation and keeps the sample chemically unaltered across the pass. For workflows requiring validated sterile-grade performance, point-of-use retention meets it without a separate sterilization step.

Membrane Characteristics

• Absolute pore-size rating holds consistent across every protocol
• Uniform capillary pore array gives high pore density without lateral crossover
• Inorganic ceramic structure resists solvent swelling common to polymer membrane materials
• Mineral oxide chemistry gives proteins little to adsorb onto
• Low intrinsic autofluorescence limits background signal in fluorescence work

Housing Characteristics

• Medical-grade polypropylene with broad chemical compatibility
• Polypropylene housing tolerates ethylene oxide gas sterilization cycles without softening or dimensional distortion.
• Minimal intrinsic autofluorescence from the polypropylene housing supports background-sensitive fluorescence-based assay work downstream.
• Minimal ionic background from the polypropylene housing supports ion chromatography and trace-metal ICP-MS sample preparation.

Filtering buffers, reagents, and aqueous samples keeps your preparation moving and consistent. General-purpose aqueous filtration supports the sample types you handle every day across your water-analysis and general analytical workflows. Bulk pricing kicks in on larger filtration orders, so ask the Tisch Scientific team for a quote.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

6.2 bar (90 psi)

Burst Pressure (psi)

100psi

Connection Type

Luer-Lok/ Luer Slip

Construction

Anopore (Aluminum Oxide)

Diameter

25mm

Filtration Area

4.15cm²

Holdup Volume

<150µl

Material

Anopore

Maximum Operating Pressure

100psi

Maximum Operating Temperature (°C)

40°C

Pack Count

50

Pore Size (µm)

0.1

Prefilter (Yes/No)

No

Sterility

Nonsterile

Volume Throughput (ml)

Up to 100ml

Wettability

Hydrophilic

• Epifluorescence Counting - Counts bacteria and viruses precisely in water samples, using the low-autofluorescence surface for microscopy.
• Gold Nanoparticle Prep - Removes large aggregate clusters from gold and silver nanoparticle suspensions before UV-Vis extinction and TEM sizing.
• Single-Particle Analysis - Concentrates nanoparticles from dilute suspensions for tunable resistive pulse sensing and nanoparticle tracking.
• Polymer Removal - Remove high molecular weight polymers efficiently, utilizing the unique inorganic membrane structure for difficult separations.
• Flow Cytometry Prep - Removes residual debris and aggregates from cell suspension buffers before immunophenotyping and bead-based multiplex assays.
• Environmental DNA Sampling - Concentrates aquatic environmental DNA from water samples for downstream eDNA metabarcoding and biodiversity monitoring.