Anotop 25mm Sterile Syringe Filters, 0.2um, 50 Pk
Whatman SKU: 6809-2122

Whatman Anotop Aluminum Oxide Syringe Filters, 0.2 µm, 25 mm, 50/Pk - 6809-2122

Whatman's Anotop line separates by physical particle dimension alone, a capability rooted in ceramic media chemistry rather than the organic polymer chemistries used in most other membrane technologies. At the sub-micron pore rating, a syringe filter delivers sterilizing-grade retention at the point of use. Inert ceramic aluminum oxide stays compatible with aqueous samples, alcohols, and organic solvents while giving proteins a short, direct channel path and provides sterile-grade retention with high recovery. The PP housing is factory-sterilized and individually wrapped for aseptic sample preparation and sustains efficient flow on viscous samples. When sample preparation calls for a sterile filtrate at the point of injection, sterilizing-grade absolute retention delivers it directly, protecting the analytical method from microbial interference.

Membrane Characteristics

• Absolute pore-size rating holds consistent across every protocol
• Chemical stability in aqueous solutions spans a pH 3 to 9 range
• Low intrinsic autofluorescence limits background signal in fluorescence work
• Extractable content stays minimal without interfering with downstream detection
• Naturally hydrophilic ceramic surface wets without pre-wetting in aqueous samples

Housing Characteristics

• Plasticizer-free polyolefin construction keeps the fluid path clean
• Polypropylene density near 0.90 g/cm3 keeps housing weight low relative to denser engineering plastics
• Each housing is factory-sterilized and sealed individually to maintain sterility through the supply chain.
• Medical-grade polypropylene with broad chemical compatibility

Upstream clarification pays for itself by keeping your columns clear and your analytical day on schedule. Upstream sample clarification keeps your separations clean and reproducible across your HPLC and UHPLC methods. Ask our friendly customer service team to confirm the pore size, membrane, and sterility options your filtration application calls for.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

6.2 bar (90 psi)

Burst Pressure (psi)

100psi

Connection Type

Luer-Lok/ Luer Slip

Construction

Anopore (Aluminum Oxide)

Diameter

25mm

Filtration Area

4.15cm²

Holdup Volume

<150µl

Material

Anopore

Maximum Operating Pressure

100psi

Maximum Operating Temperature (°C)

40°C

Pack Count

50

Pore Size (µm)

0.2

Prefilter (Yes/No)

No

Sterility

Nonsterile

Volume Throughput (ml)

Up to 100ml

Wettability

Hydrophilic

• Membrane Protein Research - Filters detergent-solubilized protein preparations to remove insoluble aggregates before crystallography and cryo-EM.
• Electron Microscopy Prep - Image retained particulates directly, using the electron-beam-stable ceramic surface for SEM and TEM sample preparation.
• Gravimetric Solids Testing - Determine particulate mass accurately, using a non-hygroscopic ceramic that resists moisture-driven drift.
• Environmental DNA Sampling - Concentrates aquatic environmental DNA from water samples for downstream eDNA metabarcoding and biodiversity monitoring.
• Multiplex Bead Assay Prep - Clears immunoassay sample matrices of residual debris that would cause carryover interference between bead populations.
• AFM Sample Deposition - Removes large aggregates from colloidal suspensions before deposition on mica for atomic force microscopy surface imaging.