Anotop Plus 25mm Syringe Filter w/GMF PreFilter, 0.02 um, 50 Pk
Whatman SKU: 6809-4002

Whatman Anotop Aluminum Oxide Syringe Filters, 0.02 µm, 25 mm, 50/Pk - 6809-4002

Whatman's Anotop line separates by physical particle dimension alone, a capability rooted in ceramic media chemistry rather than the organic polymer chemistries used in most other membrane technologies. A syringe filter dispenses filtrate directly into an autosampler vial, without the intermediate collection vessel a bulk vacuum filtration setup typically needs. The alumina membrane achieves bacterial retention at the rated pore size while limiting bacterial attachment through enhanced electrostatic repulsion at the nanoscale and tolerates high temperatures and broad solvent ranges. Thermal-bonded PP construction leaves no seam for sample to bypass the membrane and sheds virtually no extractable material into the sample. When method reproducibility rests on consistent sample prep, syringe filtration holds that standard injection after injection.

Membrane Characteristics

• Inorganic ceramic structure resists solvent swelling common to polymer membrane materials
• Low intrinsic autofluorescence limits background signal in fluorescence work
• Uniform capillary pore array gives high pore density without lateral crossover
• Chemical stability in aqueous solutions spans a pH 3 to 9 range
• Extractable content stays minimal without interfering with downstream detection

Housing Characteristics

• Translucent housing wall allows visual confirmation of sample flow during filtration
• Dilute aqueous acids, bases, and alcohols used in sample prep do not degrade the polypropylene housing
• Minimal ionic background from the polypropylene housing supports ion chromatography and trace-metal ICP-MS sample preparation.
• Standardized luer connection geometry provides secure engagement with standard syringe tips

Filtering buffers, reagents, and aqueous samples keeps your preparation moving and consistent. Consistent aqueous clarification clears particulates without slowing your bench routine the same way every time you run it. Review our Chemical Compatibility Chart, or ask technical service to confirm chemical compatibility for your specific application.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

6.9 bar (100 psi)

Burst Pressure (psi)

100psi

Connection Type

Luer-Lok/ Luer Slip

Construction

Anopore (Aluminum Oxide)

Diameter

25mm

Filtration Area

4.15cm²

Holdup Volume

<150µl

Material

Anopore

Maximum Operating Pressure

100psi

Maximum Operating Temperature (°C)

40°C

Pack Count

50

Pore Size (µm)

0.02

Prefilter (Yes/No)

No

Sterility

Nonsterile

Volume Throughput (ml)

Up to 100ml

Wettability

Hydrophilic

• Quantum Dot Preparation - Removes oversized aggregates from quantum dot suspensions ahead of photoluminescence and fluorescence spectroscopy.
• NMR Sample Filtration - Delivers aggregate-free aqueous filtrates with no organic extractables interfering with proton or carbon NMR spectra.
• Cold Sterilization - Sterilize heat-sensitive additives safely, using 0.02 µm pores to remove bacteria without degrading critical components.
• Electrolyte Clarification - Removes colloidal particles from electrochemical solutions for reproducible impedance and conductivity measurements.
• Environmental DNA Sampling - Concentrates aquatic environmental DNA from water samples for downstream eDNA metabarcoding and biodiversity monitoring.
• Flow Cytometry Prep - Removes residual debris and aggregates from cell suspension buffers before immunophenotyping and bead-based multiplex assays.