Anotop Plus 25mm Syringe Filter w/GMF PreFilter, 0.2um, 50 Pk
Whatman SKU: 6809-4022

Whatman Anotop Aluminum Oxide Syringe Filters, 0.2 µm, 25 mm, 50/Pk - 6809-4022

Whatman's Anotop line separates by physical particle dimension alone, a capability rooted in ceramic media chemistry rather than the organic polymer chemistries used in most other membrane technologies. A sterilizing-grade syringe filter carries manufacturer bacterial retention testing built into the design, giving analysts documented sterile-grade performance without repeating that testing in-house for the format. Inert ceramic aluminum oxide stays compatible with aqueous samples, alcohols, and organic solvents while giving proteins a mineral surface, not an organic one and pulls liquid the membrane's full depth at once. Polypropylene construction resists chemical degradation from routine sample matrices and holds its seal true run after run. Where bacterial contamination must stay out of the sample, manufacturer-tested retention keeps the instrument and downstream assay protected without a housing to maintain or clean.

Membrane Characteristics

• Inorganic ceramic structure resists solvent swelling common to polymer membrane materials
• Rigid inorganic structure withstands high temperatures with minimal extractables
• Chemical stability in aqueous solutions spans a pH 3 to 9 range
• Membrane surface turns optically transparent once wetted with liquid
• Uniform capillary pore array gives high pore density without lateral crossover

Housing Characteristics

• Inert polyolefin wall contributes minimal sample adsorption to the filtrate
• Low moisture absorption keeps the housing dimensionally stable through humid storage and shipping
• Thermal-bonded construction keeps adhesives out of the fluid path
• The seal formed against the membrane blocks bypass at rated operating pressure

Sample clarification before injection is the cheapest insurance your chromatography system has. Upstream sample clarification keeps your separations clean and reproducible across your HPLC and UHPLC methods. Ask how Whatman's track record as a proven brand supports standardizing your filtration workflow.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

6.2 bar (90 psi)

Burst Pressure (psi)

100psi

Connection Type

Luer-Lok/ Luer Slip

Construction

Anopore (Aluminum Oxide)

Diameter

25mm

Filtration Area

4.15cm²

Holdup Volume

<150µl

Material

Anopore

Maximum Operating Pressure

100psi

Maximum Operating Temperature (°C)

40°C

Pack Count

50

Pore Size (µm)

0.2

Prefilter (Yes/No)

No

Sterility

Nonsterile

Volume Throughput (ml)

Up to 100ml

Wettability

Hydrophilic

• Low Background Specs - Prepare samples for spectrophotometry, ensuring extremely low optical background for the most sensitive analytical readings.
• Polymer Removal - Remove high molecular weight polymers efficiently, utilizing the unique inorganic membrane structure for difficult separations.
• Electron Microscopy Prep - Image retained particulates directly, using the electron-beam-stable ceramic surface for SEM and TEM sample preparation.
• Liposome Extrusion - Size liposomes into uniform vesicles precisely, enabling consistent drug delivery research with defined pore structures.
• Quantum Dot Preparation - Removes oversized aggregates from quantum dot suspensions ahead of photoluminescence and fluorescence spectroscopy.
• Radiotracer Sample Prep - Filters aqueous radiotracer solutions with minimal ionic background for scintillation and gamma spectrometry counting.