Pura Disc 25mm PVDF Syringe Filters, 0.2um, 200 Pk
Whatman SKU: 6747-2502

Whatman Puradisc PVDF Syringe Filters, 0.2 µm, 25 mm, 200/Pk - 6747-2502

Whatman standardizes its manufacturing platform to the point that consistency and thorough lot-level records come as a default, exactly what analytical laboratory work demands. A sterilizing-grade syringe filter carries manufacturer bacterial retention testing built into the design, giving analysts documented sterile-grade performance without repeating that testing in-house for the format. PVDF achieves sterile-grade retention at sub-micron pore sizes and holds up to alcohols and mild hydrocarbon solvents. The polypropylene housing presses snugly against the membrane during the pass and stands firm against aqueous acids, bases, and alcohols during regular handling. The bench setup stays free of autoclave cycles and housing-maintenance steps once a single-use sterilizing-grade filter handles the preparation.

Technical Specifications

• Adsorptive loss of HPLC analytes stays immeasurably low
• Bacterial challenge testing with Brevundimonas diminuta at 10 million CFU per cm2 confirms sterilizing grade
• Low extractables preserve a clean chromatographic baseline
• Aqueous and HPLC samples wet the surface without pre-wetting
• Absolute pore rating ensures reproducible results between test runs

Housing Specifications

• Rigid polypropylene body maintains its geometry through routine filtration pressure
• Plasticizer-free polyolefin construction keeps the fluid path clean
• Minimal intrinsic autofluorescence from the polypropylene housing supports background-sensitive fluorescence-based assay work downstream.
• Minimal extractable release from the housing supports sensitive analytical work downstream

Upstream clarification pays for itself by keeping your columns clear and your analytical day on schedule. Upstream sample clarification keeps your separations clean and reproducible so your filtrate reaches the column clean. Ask how Whatman's track record as a proven brand supports standardizing your filtration workflow.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

1.8 bar (26 psi)

Burst Pressure (psi)

75psi

Connection Type

Luer-Lok/ Luer Slip

Construction

Polypropylene

Diameter

25mm

Filtration Area

4.2cm²

Holdup Volume

<100µl

Material

Polyvinylidene Difluoride (PVDF)

Maximum Operating Pressure

75psi

Maximum Operating Temperature (°C)

121°C

Pack Count

200

Pore Size (µm)

0.2

Prefilter (Yes/No)

No

Sterility

Nonsterile

Sterilization

Autoclavable 121°C

Volume Throughput (ml)

Up to 100ml

Wettability

Hydrophobic

• Microbiome Sampling - Concentrates waterborne microbial cells on a low-binding membrane ahead of DNA extraction and metagenomics sequencing.
• Spectroscopy Prep - Provides optical clarity and low background interference, ideal for UV-Vis and fluorescence sample preparation work.
• Trace Analysis - Prevents non-specific binding in trace analysis, keeping low-concentration analytes in solution for reliable detection.
• Forensic Toxicology Screening - Clarifies urine samples ahead of LC-MS drug screening, supporting reliable detection of abused drugs and metabolites.
• Pharmaceutical QC - Supports routine quality control testing by reliably filtering mild organic solvent mixtures and drug formulations daily.
• Protein Purification - Facilitates routine clarification of chromatography samples, preventing column fouling without binding target proteins.