Pura Disc 25mm PVDF Syringe Filters, 0.45um, 1000 Pk
Whatman SKU: 6749-2504

Whatman Puradisc PVDF Syringe Filters, 0.45 µm, 25 mm, 1000/Pk - 6749-2504

Whatman verifies performance ratings at the lot level using process controls that stay uniform across its entire filtration lineup. Because each syringe filter is dedicated to one sample, a failed or contaminated filtration never delays the next analyst's run waiting on a shared holder. PVDF holds consistent absolute retention at the rated pore size while wetting HPLC samples despite a hydrophobic fluoropolymer backbone underneath and keeps HPLC analytes from adhering to the pore surface. Polyolefin construction sheds virtually no extractable material into the sample while holding its form through high-temperature sample preparation conditions and maintains a low-dead-volume internal flow path. When method reproducibility rests on consistent sample prep, syringe filtration holds that standard injection after injection.

Technical Specifications

• Adsorptive loss of HPLC analytes stays immeasurably low
• Hydrochloric, dilute nitric, and up to 90% sulfuric acid cause no attack
• Thin membrane structure sustains high flow for fast sample throughput
• Aqueous and HPLC samples wet the surface without pre-wetting
• Organic solvent resistance beats PES or nylon at comparable pore size

Housing Specifications

• Minimal intrinsic autofluorescence from the polypropylene housing supports background-sensitive fluorescence-based assay work downstream.
• Plasticizer-free polyolefin construction keeps the fluid path clean
• Dilute aqueous acids, bases, and alcohols used in sample prep do not degrade the polypropylene housing
• Burst pressure rating provides a wide safety margin above the rated maximum operating pressure.

Clarify your routine aqueous samples in a single pass and cut down on juggling multiple filter types at the bench. Consistent aqueous clarification clears particulates without slowing your bench routine across the buffers and reagents your day-to-day prep depends on. Request Whatman's lot documentation and performance history to support your protocol validation.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

1.2 bar (17 psi)

Burst Pressure (psi)

75psi

Connection Type

Luer-Lok/ Luer Slip

Construction

Polypropylene

Diameter

25mm

Filtration Area

4.2cm²

Holdup Volume

<100µl

Material

Polyvinylidene Difluoride (PVDF)

Maximum Operating Pressure

75psi

Maximum Operating Temperature (°C)

121°C

Pack Count

1000

Pore Size (µm)

0.45

Prefilter (Yes/No)

No

Sterility

Nonsterile

Sterilization

Autoclavable 121°C

Volume Throughput (ml)

Up to 100ml

Wettability

Hydrophobic

• Mycotoxin Screening - Clarifies grain and food extracts ahead of HPLC analysis, supporting reliable detection of aflatoxins and other mycotoxins.
• Trace Analysis - Prevents non-specific binding in trace analysis, keeping low-concentration analytes in solution for reliable detection.
• Biological Samples - Clarifies protein-rich solutions without loss of target molecules, preserving sample integrity for critical downstream assays.
• Water Quality Monitoring - Clarifies environmental and drinking water samples ahead of turbidity, ion, or contaminant testing in monitoring programs.
• Spectroscopy Prep - Provides optical clarity and low background interference, ideal for UV-Vis and fluorescence sample preparation work.
• HPLC Prep - Delivers high flow rates for mild organic HPLC mobile phases, ensuring clean baselines and protecting analytical columns.