Pura Disc 25mm Nylon Syringe Filters, 0.2um, 1000 Pk
Whatman SKU: 6753-2502

Whatman Puradisc Nylon Syringe Filters, 0.2 µm, 25 mm, 1000/Pk - 6753-2502

Whatman verifies performance ratings at the lot level using process controls that stay uniform across its entire filtration lineup. Sterilizing-grade syringe filters deliver sterile-grade retention as a single-use format, eliminating the autoclaving and cleaning cycle a reusable sterile filtration setup would require. Nylon provides sterile-grade retention at sub-micron pore size while wetting on contact with aqueous buffers and fits routine analytical sample preparation. The polypropylene housing presses snugly against the membrane during the pass and sustains its dimensional form through high-temperature filtration workflows. Where bacterial contamination must stay out of the sample, manufacturer-tested retention keeps the instrument and downstream assay protected without a housing to maintain or clean.

Technical Specifications

• DMSO compatibility extends across sample-preparation chemistries
• Hydrophilic surface takes up aqueous samples with no pre-wetting
• Absolute pore size rating holds consistent across every protocol
• Low extractable contribution to sensitive analytical preparations
• Saturation moisture uptake reaches up to 8% by resin weight

Housing Specifications

• Withstands standard 121°C steam autoclave cycles without softening or structural distortion
• Minimal intrinsic autofluorescence from the polypropylene housing supports background-sensitive fluorescence-based assay work downstream.
• PP housing rated to 121°C holds its geometry through high-temperature sample preparation and processing.
• Optimized internal geometry minimizes backpressure in service

Sample clarification before injection is the cheapest insurance your chromatography system has. Upstream sample clarification keeps your separations clean and reproducible across your HPLC and UHPLC methods. Reach out to the Tisch Scientific team for application support and volume pricing on your filtration product selection.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

2.9 bar (42 psi)

Burst Pressure (psi)

75psi

Connection Type

Luer-Lok/ Luer Slip

Construction

Polypropylene

Diameter

25mm

Filtration Area

4.2cm²

Holdup Volume

<100µl

Material

Nylon

Maximum Operating Pressure

75psi

Maximum Operating Temperature (°C)

121°C

Pack Count

1000

Pore Size (µm)

0.2

Prefilter (Yes/No)

No

Sterility

Nonsterile

Sterilization

Autoclavable 121°C

Volume Throughput (ml)

Up to 100ml

Wettability

Hydrophilic

• Water Quality Monitoring - Removes suspended solids from environmental water samples for routine total suspended solids testing.
• Amino Acid Profiling - Clarifies aqueous sample digests for HPLC and LC-MS amino acid analysis in food, nutrition, and fermentation monitoring.
• GC Sample Prep - Clarifies aqueous and organic extracts ahead of gas chromatography injection, protecting inlets from particulate fouling.
• Dissolution Testing - Supports accurate drug release profiles by clarifying dissolution samples ahead of HPLC quantitation in QC labs.
• Environmental Extract Prep - Clarifies water and soil extracts ahead of trace metals or pesticide-residue analysis for accurate results.
• QuEChERS Cleanup - Clarifies acetonitrile food extracts after dispersive cleanup, protecting GC-MS and LC-MS systems during pesticide screening.