Pura Disc 25mm PES Syringe Filters, 0.45um, 50 Pk
Whatman SKU: 6780-2504

Whatman Puradisc Polyethersulfone (PES) Syringe Filters, 0.45 µm, 25 mm, 50/Pk - 6780-2504

Whatman has built such a strong track record in published methods that laboratories default to citing its name in standardized protocols. A syringe filter's small hold-up volume limits sample loss, a meaningful difference when working with a limited or precious sample. PES pairs fast flow with reduced protein and antibody adsorption and admits aqueous reagents on contact, skipping any surfactant rinse. The polypropylene housing resists aqueous acids, bases, and alcohols in routine use and presses snugly against the membrane during the pass. For routine sample preparation across many methods, each analytical run starts with a consistent, particle-free sample without added hardware or a cleaning step.

Technical Specifications

• DNA and RNA pass through with negligible adsorptive loss
• High flow rate pairs with a low extractable background
• Pore structure integrity holds through autoclave cycles up to 126°C
• Non-hydrogen-bonding surface avoids the polar adsorption nylon's amide backbone causes
• Absolute pore size rating holds consistent across every lot

Housing Specifications

• Minimal intrinsic autofluorescence from the polypropylene housing supports background-sensitive fluorescence-based assay work downstream.
• Thermal-bonded construction keeps adhesives out of the fluid path
• The seal formed against the membrane blocks bypass at rated operating pressure
• Dilute aqueous acids, bases, and alcohols used in sample prep do not degrade the polypropylene housing

Filtering buffers, reagents, and aqueous samples keeps your preparation moving and consistent. General-purpose aqueous filtration supports the sample types you handle every day across your water-analysis and general analytical workflows. Start a live chat with our customer support technicians for real-time updates on product availability, lead time, or your order status.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

2.3 bar (33 psi)

Burst Pressure (psi)

75psi

Connection Type

Luer-Lok/ Luer Slip

Construction

Polypropylene

Diameter

25mm

Filtration Area

4.2cm²

Holdup Volume

<100µl

Material

Polyethersulfone (PES)

Maximum Operating Pressure

75psi

Maximum Operating Temperature (°C)

121°C

Pack Count

50

Pore Size (µm)

0.45

Prefilter (Yes/No)

No

Sterility

Nonsterile

Sterilization

Autoclavable 121°C

Volume Throughput (ml)

Up to 100ml

Wettability

Hydrophilic

• Serum Filtration - Clarifies serum and plasma samples quickly while retaining critical proteins needed for accurate downstream analytical testing.
• Nucleic Acid Recovery - Processes DNA and RNA extracts with minimal nucleic acid adsorption, preserving yield on the hydrophilic PES surface.
• Pesticide Residue Testing - PES membranes improve polar pesticide recovery in food-safety LC-MS/MS testing versus hydrogen-bonding nylon.
• Organic Carbon Testing - Clarifies water samples ahead of DOC and UV254 analysis while keeping organic carbon adsorption to the membrane minimal.
• Life Science Prep - Speeds processing of viscous biological solutions, improving laboratory workflow efficiency during busy sample prep days.
• Biological Assays - Protects sensitive enzymatic and antibody-based assays from particulate interference without adsorbing critical reagents.