Pura Disc 25mm PES Syringe Filters, 0.45um, 1000 Pk
Whatman SKU: 6794-2504

Whatman Puradisc Polyethersulfone (PES) Syringe Filters, 0.45 µm, 25 mm, 1000/Pk - 6794-2504

Whatman has built such a strong track record in published methods that laboratories default to citing its name in standardized protocols. Across analytical workflows, the syringe filter is where particulates leave the sample stream. PES's open pore layout leaves proteins less surface to contact and tolerates aliphatic alcohols, glycols, and aqueous buffers. Polypropylene construction resists chemical degradation from routine sample matrices and maintains a low-dead-volume internal flow path. When chromatographic methods demand particle-free injections, point-of-use syringe filtration clarifies the sample before it reaches the column.

Technical Specifications

• PES's aromatic sulfone backbone gives the membrane enough hydrolytic stability to tolerate autoclave steam sterilization cycles
• PES membrane's compatibility with nucleic acid filtration extends its use beyond protein-based sample preparation
• PES's non-ionic surface avoids the charge-driven protein binding characteristic of positively and negatively charged membrane types
• PES membranes show non-detectable PFAS extractables under EPA Method 537.1, supporting ultra-trace environmental water analysis
• Sterilizing-grade PES membrane lots are challenge-tested against Brevundimonas diminuta to qualify 0.2 micron retention

Housing Specifications

• Minimal intrinsic autofluorescence from the polypropylene housing supports background-sensitive fluorescence-based assay work downstream.
• Dilute aqueous acids, bases, and alcohols used in sample prep do not degrade the polypropylene housing
• Stable across the full aqueous pH range in routine sample work
• Plasticizer-free polyolefin construction keeps the fluid path clean

Filtering buffers, reagents, and aqueous samples keeps your preparation moving and consistent. General-purpose aqueous filtration supports the sample types you handle every day across your water-analysis and general analytical workflows. Standardize your filtration purchases across every method with volume pricing and documentation from the Tisch Scientific team.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

2.3 bar (33 psi)

Burst Pressure (psi)

75psi

Connection Type

Luer-Lok/ Luer Slip

Construction

Polypropylene

Diameter

25mm

Filtration Area

4.2cm²

Holdup Volume

<100µl

Material

Polyethersulfone (PES)

Maximum Operating Pressure

75psi

Maximum Operating Temperature (°C)

121°C

Pack Count

1000

Pore Size (µm)

0.45

Prefilter (Yes/No)

No

Sterility

Nonsterile

Sterilization

Autoclavable 121°C

Volume Throughput (ml)

Up to 100ml

Wettability

Hydrophilic

• Urine Drug Screening - Clarifies protein-poor urine samples for dilute-and-shoot LC-MS/MS toxicology testing without adsorbing target drug analytes.
• Nucleic Acid Recovery - Processes DNA and RNA extracts with minimal nucleic acid adsorption, preserving yield on the hydrophilic PES surface.
• Biological Assays - Protects sensitive enzymatic and antibody-based assays from particulate interference without adsorbing critical reagents.
• Organic Carbon Testing - Clarifies water samples ahead of DOC and UV254 analysis while keeping organic carbon adsorption to the membrane minimal.
• Pesticide Residue Testing - PES membranes improve polar pesticide recovery in food-safety LC-MS/MS testing versus hydrogen-bonding nylon.
• Biological Fluids - Filters buffers and biological fluids efficiently, using the PES membrane to maximize flow and minimize non-specific binding.