Hydrophilic PVDF Membrane Filters, 0.45um, 250mm, Nonsterile, 50/Pk SKU: SF18140

PVDF Membrane Filters - SF18140

Designed to deliver reproducibility and confidence across critical sample types, the Tisch SF18140 provides the filtration consistency that method validation demands. Verified 0.45 pore distribution across the hydrophilic membrane surface guarantees batch-to-batch consistency in particle capture and analyte passage. Each membrane disc reflects a manufacturing standard where retention characteristics, physical integrity, and dimensional accuracy are verified before release.

Material Specifications

• Oxidation and UV stability allow PVDF membranes to retain their mechanical and chemical properties through prolonged storage and repeated use.
• Economical bulk packaging supports laboratories with high consumable throughput where individual sterile wrapping is unnecessary.
• Spontaneous aqueous wettability reduces sample preparation time and eliminates the risk of solvent carryover from pre-wetting procedures.
• High thermal stability allows PVDF membranes to maintain structural integrity during autoclaving and elevated-temperature processing cycles.
• Rated for continuous operation at temperatures reaching 80°C, enabling filtration of heated solvents and process fluids without material compromise.

When your analytical method specifies membrane filtration as a sample preparation step, the retention characteristics and extractable profiles documented above allow you to meet method requirements without additional qualification work. Meeting this challenge, ultra-low protein binding on the modified PVDF surface preserves analyte concentrations in your biological filtrates, protecting the yield your downstream assays depend on. Secure your research outcomes by partnering with a filtration solution that understands your need for speed, accuracy, and reproducibility.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

≥21.8 psi (water, 25°C)

Capacity

30mL

Color

White

Diameter

250mm

Extractables

Low

Filtration Area

490.87cm²

Grid (Yes/No)

No

Material

Polyvinylidene Fluoride (PVDF)

Maximum Operating Temperature (°C)

80°C

Pack Count

50

pH Range

1-12pH

Pore Size (µm)

0.45

Sterility

Nonsterile

Sterilization

Autoclave, EtO, Gamma Irradiation

Weight

0.65

Wettability

Hydrophilic

• Colony Lifts - Transfers bacterial colonies from agar plates for hybridization screening, maintaining the exact spatial pattern of growth.
• Western Blot Transfer - The gold standard for protein transfer, offering high binding capacity and physical strength for immunoblotting assays.
• Immunodetection Assay - Provides a high-sensitivity background for colorimetric detection, enhancing the contrast of faint protein bands.
• Protein Dot Blotting - Facilitates the rapid screening of multiple protein samples by applying them directly to the membrane grid surface.
• Amino Acid Analysis - Used for protein sequencing where the membrane must withstand harsh chemical degradation steps without dissolving away.
• Solid Phase Sequencing - Immobilizes proteins securely for Edman degradation cycles, ensuring the polypeptide chain remains fixed during wash steps.