M-FC Broth with Rosolic Acid Ampoules, 2mL, Nonsterile Packaging, 50/Pk
GVS SKU: 10496114

Featuring a ready-to-use, reddish-purple liquid medium dispensed in single-use 2mL plastic ampoules, this M-FC Broth with Rosolic Acid supports the detection and enumeration of fecal coliforms in water and other liquid samples using the membrane filtration technique. The medium relies on the property that fecal coliforms grow at elevated temperatures near 44.5C, providing a highly selective and differential environment for water quality and wastewater testing. Each 2mL ampoule holds enough medium to saturate one standard absorbent pad in a petri dish, and the medium is supplied in packs of 50 ampoules.

Key Media Characteristics

• Rosolic acid serves as the differential indicator system, helping distinguish fecal coliforms from other organisms.
• Bile salts inhibit the growth of Gram-positive bacteria for added selectivity.
• Fecal coliforms ferment lactose at elevated temperature and form blue colonies, while non-coliforms form gray to cream-colored colonies.
• Enzymatic digest of animal tissue, yeast extract, sodium chloride, and lactose provide nutrients, vitamins, osmotic balance, and a carbohydrate source.
• Reddish-purple liquid format is pre-portioned for consistent, reliable results.

Key Construction Characteristics

• Supplied in 2mL plastic ampoules that simplify pouring and avoid the manipulation of glass.
• Each ampoule delivers a single-use, pre-measured volume sufficient to saturate one absorbent pad.
• Packaged 50 ampoules per pack, sealed in a labeled foil bag.
• Product is supplied sterilized and is temperature sensitive, requiring storage at the recommended temperature.

This ready-to-use liquid medium streamlines microbiology workflows where time, equipment, and trained personnel may be limited. The pre-portioned ampoule format reduces preparation time and the risk of cross-contamination, delivering a clear visual result for rapid and accurate fecal coliform testing. It is well suited for assessing waste and effluent waters and routine water quality monitoring.

Capacity

2 mL

Color

Reddish-purple

Compliance Standards

ISO 9001

Material

M-FC Broth with Rosolic Acid

Maximum Operating Temperature (°C)

44.5

Pack Count

50

Sterility

Nonsterile

• Fecal Coliform Enumeration Supports detection and enumeration of fecal coliforms in water and other liquid samples using the membrane filtration technique.
• Wastewater Testing Recommended for testing waste and effluent waters for the presence of fecal coliforms.
• Water Quality Monitoring Used to assess ground, surface, recreational, and potable water sources for microbiological safety.
• Membrane Filtration Workflows Each 2mL ampoule saturates one standard absorbent pad in a petri dish for use with membrane filters.
• Differential Coliform Identification Produces blue colonies for fecal coliforms and gray to cream-colored colonies for other organisms at 44.5C incubation.