PROTRAN BA83 82mm 50/Pk SKU: 10401316

Protran Nitrocellulose Blotting Membranes - 10401316

Capturing proteins across a wide molecular weight range during electrophoretic transfer requires a membrane with the surface chemistry and pore structure optimized for hydrophobic protein immobilization. Low-extractable nitrocellulose formulation produces less than 1.0% extractables, meeting the purity requirements for cell growth, tissue culture, and sensitive immunodetection applications. Optimized surface chemistry ensures efficient biomolecule capture and clear signal generation for publication-quality blotting results.

Membrane Specifications

• Protran transfer membranes offer the binding capacity and low background performance that molecular biology laboratories require for routine immunoblotting, dot and slot blotting, and colony and plaque lift applications.
• Pure 100% nitrocellulose composition without cellulose acetate additives ensures the highest possible protein binding capacity across all molecular weight ranges.
• Neutral surface chemistry captures proteins via hydrophobic binding mechanisms that preserve native conformation and long-term molecular recognition activity.
• Selected 0.2μm pore rating optimizes retention characteristics for your target molecular weight range during electrophoretic or capillary transfer.
• Manufactured from 100% pure nitrocellulose without cellulose acetate additives, Protran membranes deliver binding capacity of 80–100 μg/cm² and superior signal-to-noise ratios for protein immunodetection across all common detection platforms.

If your laboratory runs standardized blotting protocols across multiple analysts or shifts, the dimensional precision and binding uniformity of this membrane reduce the operator-to-operator variability that can compromise your data quality. Simplified preparation workflow requiring only water or buffer pre-wetting reduces hands-on time and eliminates methanol exposure during membrane activation. If your laboratory requires volume pricing, custom packaging configurations, or scheduled delivery programs, the Tisch Scientific sales team can structure a supply arrangement matched to your consumption rate.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Diameter

82mm

Material

Nitrocellulose (NC)

Sterility

Nonsterile

Wettability

Hydrophilic

• Lateral Flow Assay Development — Nitrocellulose test strip fabrication for point-of-care diagnostics requiring stable protein immobilization.
• Colony and Plaque Lifts — In situ transfer of bacterial colonies or phage plaques for hybridization-based screening of genomic and cDNA libraries.
• Dot and Slot Blotting — Direct application of protein or nucleic acid samples for rapid semi-quantitative screening without gel separation.
• DNA-Protein Interaction Studies — Immobilization of protein targets for Southwestern blotting and DNA-binding domain characterization experiments.
• Protein Immunodetection — Chemiluminescent, colorimetric, and fluorescent visualization of antibody-bound targets for expression studies.
• Western Blotting — Electrophoretic transfer and immunodetection of target proteins separated by SDS-PAGE for expression analysis.