PROTRAN BA85 25mm 100/Pk SKU: 10402506

Protran Nitrocellulose Blotting Membranes - 10402506

Achieving reliable Western blot results demands a transfer membrane that combines exceptional protein binding with consistently low background signal across every experiment. Proprietary nitrocellulose formula maintains bound protein molecular recognition activity for up to five years, supporting long-term archival of completed blots and diagnostic reference standards. Broad detection method compatibility provides the analytical flexibility to adapt workflows as experimental requirements evolve.

Membrane Specifications

• Proprietary nitrocellulose formula preserves bound protein molecular recognition activity for up to five years of archival storage.
• Pure nitrocellulose construction gives Protran membranes the surface properties needed for superior signal-to-noise ratios without stringent washing conditions, while a proprietary formula preserves bound protein recognition activity for up to five years.
• Manufactured from 100% pure nitrocellulose without cellulose acetate additives, Protran membranes deliver binding capacity of 80–100 μg/cm² and superior signal-to-noise ratios for protein immunodetection across all common detection platforms.
• Less than 1.0% extractables meets the purity requirements for cell growth, tissue culture, and sensitive immunodetection applications.
• Engineered for Western, Southern, and Northern blotting, Protran membranes combine low background fluorescence with broad detection compatibility spanning isotopic, chemiluminescent, colorimetric, and fluorescent methods.

If your blotting workflow demands reproducible signal intensity across experimental replicates, the lot-to-lot manufacturing controls behind this membrane help ensure that your optimized conditions stay valid from one experiment to the next. Proven five-year protein shelf life on the membrane surface supports both routine blotting workflows and long-term archival of diagnostic reference blots and quality control standards. If your laboratory requires volume pricing, custom packaging configurations, or scheduled delivery programs, the Tisch Scientific sales team can structure a supply arrangement matched to your consumption rate.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Diameter

25mm

Material

Nitrocellulose (NC)

Sterility

Nonsterile

Wettability

Hydrophilic

• Western Blotting — Electrophoretic transfer and immunodetection of target proteins separated by SDS-PAGE for expression analysis.
• Southern Blotting — Capillary transfer and hybridization of restriction-digested DNA fragments for gene mapping and linkage analysis.
• Lateral Flow Assay Development — Nitrocellulose test strip fabrication for point-of-care diagnostics requiring stable protein immobilization.
• Northern Blotting — Transfer and hybridization of size-fractionated RNA for gene expression profiling and transcript size determination.
• DNA-Protein Interaction Studies — Immobilization of protein targets for Southwestern blotting and DNA-binding domain characterization experiments.
• Colony and Plaque Lifts — In situ transfer of bacterial colonies or phage plaques for hybridization-based screening of genomic and cDNA libraries.