PROTRAN BA85 25mm 100/Pk
Whatman SKU: 10402506

Nitrocellulose Blotting Membranes, 0.45 µm, 25 mm, 100/pk - 10402506

Whatman's brand recognition and documented performance history support specification of its membranes in standardized blotting and transfer protocols. High-binding nitrocellulose anchors proteins for sensitive, low-background detection. Hydrophilic nitrocellulose wets in transfer buffer without methanol activation while keeping background low for clean detection and resolves low-molecular-weight proteins with sharp bands. Where low-molecular-weight proteins must resolve sharply, nitrocellulose captures them across the membrane.

Key Characteristics

• Hydrophilic surface wets in transfer buffer without methanol activation
• Low background sharpens signal across detection methods
• High protein binding captures transferred proteins for sensitive detection
• Compatible with chemiluminescent, colorimetric, fluorescent, and radioactive detection
• Optimized for aqueous electrophoretic transfer and immunodetection

Capturing every transferred protein and holding each through detection keeps your Western results clean. Dependable protein immobilization keeps your Western blot quantitative through your blocking, probing, and imaging steps. Start a live chat with our customer support technicians to match membrane chemistry to your blotting application.

Diameter

25mm

Material

Nitrocellulose (NC)

Sterility

Nonsterile

Wettability

Hydrophilic

• Protein Immunodetection - Binds transferred proteins for antibody-based visualization across all common detection methods reliably.
• Western Blotting - Captures transferred proteins for chemiluminescent, colorimetric, and fluorescent immunodetection workflows.
• Dot and Slot Blotting - Immobilizes directly applied protein samples for rapid semi-quantitative screening and detection assays.
• Immunoassay Development - Immobilizes antigens or antibodies on nitrocellulose for membrane-based immunoassay development and testing.
• Low-MW Protein Detection - Resolves low-molecular-weight proteins with sharp bands and clean, low-background detection signal.
• Lateral Flow Strips - Anchors capture proteins on nitrocellulose for point-of-care diagnostic test strip fabrication and assembly.