PROTRAN BA85 132mm 50/PkG SKU: 10402525

Protran Nitrocellulose Blotting Membranes - 10402525

Capturing proteins across a wide molecular weight range during electrophoretic transfer requires a membrane with the surface chemistry and pore structure optimized for hydrophobic protein immobilization. Hydrophilic surface properties allow direct wetting in transfer buffer without methanol activation, preserving native protein conformation for enzymes and other activity-sensitive targets. Broad detection method compatibility provides the analytical flexibility to adapt workflows as experimental requirements evolve.

Membrane Specifications

• Designed as the universal standard for protein blotting, Protran nitrocellulose membranes provide reliable biomolecule immobilization with less than 1.0% extractables and quality-controlled lot-to-lot consistency.
• Compatible with radioactive, chemiluminescent, colorimetric, and fluorescent detection methods for flexible downstream analytical workflows.
• Pore size of 0.45μm balances efficient target capture with controlled transfer speed to minimize blow-through of lower molecular weight biomolecules.
• Proprietary nitrocellulose formula preserves bound protein molecular recognition activity for up to five years of archival storage.
• Less than 1.0% extractables meets the purity requirements for cell growth, tissue culture, and sensitive immunodetection applications.

Whether your experiments involve routine Western blots or multi-probe hybridization studies, the binding consistency and low background documented above protect the clarity of every result you publish. High protein binding capacity paired with inherently low background signal makes Protran nitrocellulose a dependable choice for laboratories running Western blot, dot blot, and immunodetection assays at any throughput level. For detailed specification sheets, validation documentation, or technical guidance on membrane selection for your specific application, the Tisch Scientific team is available to support your evaluation.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Diameter

132mm

Material

Nitrocellulose (NC)

Sterility

Nonsterile

Wettability

Hydrophilic

• Colony and Plaque Lifts — In situ transfer of bacterial colonies or phage plaques for hybridization-based screening of genomic and cDNA libraries.
• Northern Blotting — Transfer and hybridization of size-fractionated RNA for gene expression profiling and transcript size determination.
• Protein Immunodetection — Chemiluminescent, colorimetric, and fluorescent visualization of antibody-bound targets for expression studies.
• Dot and Slot Blotting — Direct application of protein or nucleic acid samples for rapid semi-quantitative screening without gel separation.
• Western Blotting — Electrophoretic transfer and immunodetection of target proteins separated by SDS-PAGE for expression analysis.
• DNA-Protein Interaction Studies — Immobilization of protein targets for Southwestern blotting and DNA-binding domain characterization experiments.