PROTRAN BA85 25mm 1000/Pk SKU: 10402578

Protran Nitrocellulose Blotting Membranes - 10402578

Successful blotting workflows depend on transfer membranes that deliver high binding capacity without the methanol pre-wetting steps and stringent washing conditions that slow laboratory throughput. Controlled pore structure delivers consistent protein retention across molecular weights, with smaller pore sizes available to prevent blow-through of low-molecular-weight targets below 20 kDa. Quality-controlled manufacturing ensures the membrane consistency that demanding blotting applications require for reproducible, defensible results.

Membrane Specifications

• Manufactured from 100% pure nitrocellulose without cellulose acetate additives, Protran membranes deliver binding capacity of 80–100 μg/cm² and superior signal-to-noise ratios for protein immunodetection across all common detection platforms.
• Protran transfer membranes offer the binding capacity and low background performance that molecular biology laboratories require for routine immunoblotting, dot and slot blotting, and colony and plaque lift applications.
• Designed as the universal standard for protein blotting, Protran nitrocellulose membranes provide reliable biomolecule immobilization with less than 1.0% extractables and quality-controlled lot-to-lot consistency.
• Compatible with radioactive, chemiluminescent, colorimetric, and fluorescent detection methods for flexible downstream analytical workflows.
• Engineered for Western, Southern, and Northern blotting, Protran membranes combine low background fluorescence with broad detection compatibility spanning isotopic, chemiluminescent, colorimetric, and fluorescent methods.

When your research timeline depends on getting defensible blotting data without repeated optimization cycles, the transfer efficiency and signal clarity built into this membrane help you move from experiment to answer with confidence. Compatibility with radioactive, chemiluminescent, colorimetric, and fluorescent detection platforms gives researchers the flexibility to adapt their detection strategy without changing the transfer membrane. For questions about chemical compatibility, operating limits, or equivalent product cross-references, Tisch Scientific's filtration specialists can provide the technical detail your method documentation requires.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Diameter

25mm

Material

Nitrocellulose (NC)

Sterility

Nonsterile

Wettability

Hydrophilic

• Northern Blotting — Transfer and hybridization of size-fractionated RNA for gene expression profiling and transcript size determination.
• Lateral Flow Assay Development — Nitrocellulose test strip fabrication for point-of-care diagnostics requiring stable protein immobilization.
• Protein Immunodetection — Chemiluminescent, colorimetric, and fluorescent visualization of antibody-bound targets for expression studies.
• Colony and Plaque Lifts — In situ transfer of bacterial colonies or phage plaques for hybridization-based screening of genomic and cDNA libraries.
• Western Blotting — Electrophoretic transfer and immunodetection of target proteins separated by SDS-PAGE for expression analysis.
• DNA-Protein Interaction Studies — Immobilization of protein targets for Southwestern blotting and DNA-binding domain characterization experiments.