PROTRAN BA85 25mm 1000/Pk
Whatman SKU: 10402578

Nitrocellulose Blotting Membranes, 0.45 µm, 25 mm, 1000/pk - 10402578

Whatman's expertise in membrane casting guides the surface chemistry and quality control behind reproducible capture of transferred molecules. High-binding nitrocellulose anchors proteins for sensitive, low-background detection. The membrane suits Western blotting and lateral flow strip fabrication and loads directly without a pre-wetting step. When a Western blot needs clean, low-background signal, nitrocellulose holds the protein through detection.

Key Characteristics

• Hydrophilic surface wets in transfer buffer without methanol activation
• Compatible with chemiluminescent, colorimetric, fluorescent, and radioactive detection
• Well suited to low-molecular-weight proteins and lateral flow strips
• Low background sharpens signal across detection methods
• Optimized for aqueous electrophoretic transfer and immunodetection

Capturing every transferred protein and holding each through detection keeps your Western results clean. Dependable protein immobilization keeps your Western blot quantitative across chemiluminescent, colorimetric, and fluorescent detection. Contact Tisch Scientific for help selecting the right blotting membrane for your transfer and detection workflow.

Diameter

25mm

Material

Nitrocellulose (NC)

Sterility

Nonsterile

Wettability

Hydrophilic

• Dot and Slot Blotting - Immobilizes directly applied protein samples for rapid semi-quantitative screening and detection assays.
• Protein Immunodetection - Binds transferred proteins for antibody-based visualization across all common detection methods reliably.
• Immunoassay Development - Immobilizes antigens or antibodies on nitrocellulose for membrane-based immunoassay development and testing.
• Lateral Flow Strips - Anchors capture proteins on nitrocellulose for point-of-care diagnostic test strip fabrication and assembly.
• Low-MW Protein Detection - Resolves low-molecular-weight proteins with sharp bands and clean, low-background detection signal.
• Western Blotting - Captures transferred proteins for chemiluminescent, colorimetric, and fluorescent immunodetection workflows.