NC45 0.45um 50mm 100/Pk SKU: 10401114

Cellulose Nitrate (CN) Membrane Filters - 10401114

Engineered for high retention efficiency and superior chemical stability, the Whatman 10401114 achieves reliable separation results across a broad range of analytical and preparative applications. Moreover, precision-manufactured Hydrophilic membrane with a verified 0.45 pore structure delivers consistent retention across the full operating range. In sum, from raw polymer through final inspection, the manufacturing process is designed to ensure that every membrane disc meets the performance specifications of its grade designation.

Material Specifications

• High protein binding capacity makes cellulose nitrate the preferred membrane for Western blotting, dot blotting, and nucleic acid immobilization techniques that rely on efficient surface adsorption.
• Naturally hydrophilic and compatible with aqueous solutions, cellulose nitrate delivers high flow rates and rapid wet-out without requiring pre-conditioning or surfactant treatment.
• Dissolves completely in specific organic solvents, enabling full recovery of captured particles from the membrane surface for downstream gravimetric, chemical, or spectroscopic analysis.
• Cellulose nitrate membranes feature a narrow pore size distribution and low extractable levels, providing the controlled retention characteristics required for gravimetric and particulate analysis.
• Autoclavable at 121°C without loss of integrity, cellulose nitrate membranes maintain their rated pore structure and dimensional stability through repeated sterilization cycles.

Reproducible particle counting and size distribution analysis start with a membrane surface that captures particles uniformly — the pore consistency documented above ensures your counts reflect the sample, not random variation in the filter. In this context, surface retention on cellulose nitrate keeps captured particles and microorganisms on the membrane face where they remain accessible for direct microscopic examination, colony counting, and physical recovery without sectioning or dissolution. Elevate the quality of your analytical data today by standardizing on a filtration product built to meet the exact specifications of your applications.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

40.6psi

Color

white

Diameter

50mm

Extractables

low

Filtration Area

19.63cm²

Flow Rate

60ml (Water)

Grid (Yes/No)

No

Material

Cellulose Nitrate (CN)

Maximum Operating Pressure

Holder-dependent

Maximum Operating Temperature (°C)

80°C

Membrane Thickness (µm)

105–140um

Pack Count

100

pH Range

4-8

Pore Size (µm)

0.45

Sterility

Nonsterile

Sterilization

Autoclavable at 121°C for 30 mins

Support Material

None

Wettability

Hydrophilic

• Bioburden Monitoring - Surface retention keeps captured organisms accessible for direct incubation and enumeration without transfer steps.
• Routine Sample Prep - Uniform microporous matrix provides consistent flow rates and reliable particle retention for general aqueous solutions.
• Biochemical Assays - High protein-binding capacity effectively immobilizes macromolecules, supporting lateral flow and diagnostic testing.
• Environmental Compliance - Documented retention characteristics at standard pore ratings support reproducibility in regulated testing programs.
• Aerosol Analysis - Low tare weight and acid digestibility enable chemical characterization of airborne particulates from air sampling.
• Gravimetric Particulate Analysis - Dissolves in organic solvents for complete particle recovery and accurate weight-based quantification.