Whatman
NC45 0.45um 50mm 100/Pk SKU: 10401114

Cellulose Nitrate (CN) Membrane Filters - 10401114

The 10401114 combines a controlled pore size distribution with low extractable levels, ensuring the data accuracy that sensitive analytical methods require. Precision membrane geometry balances retention sharpness with sustainable flow rates, minimizing the pressure buildup that leads to premature filter replacement. Controlled production conditions produce membranes with documented pore size distribution and thickness tolerances, supporting method reproducibility across multiple filter lots.

Material Specifications

• High protein binding capacity makes cellulose nitrate the preferred membrane for Western blotting, dot blotting, and nucleic acid immobilization techniques that rely on efficient surface adsorption.
• Dissolves completely in specific organic solvents, enabling full recovery of captured particles from the membrane surface for downstream gravimetric, chemical, or spectroscopic analysis.
• Bulk packaging format provides cost-effective access for high-volume laboratories performing non-critical filtration and sample preparation.
• Naturally hydrophilic and compatible with aqueous solutions, cellulose nitrate delivers high flow rates and rapid wet-out without requiring pre-conditioning or surfactant treatment.
• Thermal stability of the construction materials allows safe operation at temperatures up to 80°C without degradation of membrane or housing performance.

When your analytical method specifies membrane filtration as a sample preparation step, the retention characteristics and extractable profiles documented above allow you to meet method requirements without additional qualification work. Because cellulose nitrate dissolves completely in organic solvents like acetone and amyl acetate, you can recover every captured particle from the membrane surface for downstream gravimetric, spectroscopic, or chemical analysis. If you need help matching a membrane chemistry and pore size to your sample matrix, Tisch Scientific's technical staff can walk you through the selection process and provide application-specific recommendations.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Diameter

50mm

Pore Size (µm)

0.45

Sterility

Nonsterile

Wetability

Hydrophilic

• Quality Control Testing - Serves as a standard check filter for routine batch purity tests, verifying the absence of visible physical contaminants.
• Sediment Collection - Efficiently harvests precipitates from chemical reactions, allowing for the recovery and drying of the solid synthesis product.
• Stack Pre-filtration - Acts as a spacer or depth layer in multi-tier filtration stacks to extend the life of the final downstream membrane.
• Microscopy mounting - Retains microorganisms on a flat surface, allowing for direct staining and enumeration under a high-power microscope.
• Radiometric Counting - Collects radioactive precipitates for scintillation counting, providing a consistent geometry for accurate data measurement.
• Vacuum Clarification - Utilized in Buchner funnels to rapidly remove particulates from large-volume batches of non-critical laboratory buffers.