Whatman
NC45 0.45um 100mm 50/Pk SKU: 10401121

Cellulose Nitrate (CN) Membrane Filters - 10401121

Engineered for high retention efficiency and superior chemical stability, the 10401121 achieves reliable separation results across a broad range of analytical and preparative applications. Quality-controlled manufacturing ensures the filtration medium delivers the retention efficiency and throughput that demanding laboratory protocols require. Consistent surface properties across the full membrane area support uniform particle capture and reliable results in gravimetric, microscopic, and culture-based analytical workflows.

Material Specifications

• Thermal stability of the construction materials allows safe operation at temperatures up to 80°C without degradation of membrane or housing performance.
• Dissolves completely in specific organic solvents, enabling full recovery of captured particles from the membrane surface for downstream gravimetric, chemical, or spectroscopic analysis.
• Cellulose nitrate membranes feature a narrow pore size distribution and low extractable levels, providing the controlled retention characteristics required for gravimetric and particulate analysis.
• Surface retention characteristics allow captured particles and microorganisms to remain on the membrane face, supporting direct microscopic examination and physical recovery of collected material.
• High protein binding capacity makes cellulose nitrate the preferred membrane for Western blotting, dot blotting, and nucleic acid immobilization techniques that rely on efficient surface adsorption.

Microbial enumeration methods require complete retention at the rated pore size and a surface that supports colony growth without inhibition, and the membrane specifications above confirm both requirements for your culture-based workflows. Surface retention on cellulose nitrate keeps captured particles and microorganisms on the membrane face where they remain accessible for direct microscopic examination, colony counting, and physical recovery without sectioning or dissolution. For detailed specification sheets, validation documentation, or technical guidance on membrane selection for your specific application, the Tisch Scientific team is available to support your evaluation.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Diameter

100mm

Pore Size (µm)

0.45

Sterility

Nonsterile

Wetability

Hydrophilic

• Stack Pre-filtration - Acts as a spacer or depth layer in multi-tier filtration stacks to extend the life of the final downstream membrane.
• Quality Control Testing - Serves as a standard check filter for routine batch purity tests, verifying the absence of visible physical contaminants.
• Microscopy mounting - Retains microorganisms on a flat surface, allowing for direct staining and enumeration under a high-power microscope.
• Vacuum Clarification - Utilized in Buchner funnels to rapidly remove particulates from large-volume batches of non-critical laboratory buffers.
• Gravimetric Analysis - Captures total suspended solids from liquid samples on a weighed disc, ensuring accurate mass measurements for reporting.
• Radiometric Counting - Collects radioactive precipitates for scintillation counting, providing a consistent geometry for accurate data measurement.