WCN WH 25mm 0.45um 100/Pk SKU: 7184-002

Cellulose Nitrate (CN) Membrane Filters - 7184-002

Engineered for high retention efficiency and superior chemical stability, the Whatman 7184-002 achieves reliable separation results across a broad range of analytical and preparative applications. Verified 0.45 pore distribution across the Hydrophilic membrane surface guarantees batch-to-batch consistency in particle capture and analyte passage. Together, controlled production conditions produce membranes with documented pore size distribution and thickness tolerances, supporting method reproducibility across multiple filter lots.

Material Specifications

• Surface retention characteristics allow captured particles and microorganisms to remain on the membrane face, supporting direct microscopic examination and physical recovery of collected material.
• Naturally hydrophilic and compatible with aqueous solutions, cellulose nitrate delivers high flow rates and rapid wet-out without requiring pre-conditioning or surfactant treatment.
• High protein binding capacity makes cellulose nitrate the preferred membrane for Western blotting, dot blotting, and nucleic acid immobilization techniques that rely on efficient surface adsorption.
• Autoclavable at 121°C without loss of integrity, cellulose nitrate membranes maintain their rated pore structure and dimensional stability through repeated sterilization cycles.
• Cellulose nitrate membranes feature a narrow pore size distribution and low extractable levels, providing the controlled retention characteristics required for gravimetric and particulate analysis.

If your protocol requires direct on-membrane analysis by microscopy or culture, the surface characteristics and dimensional tolerances described above give you a substrate that performs consistently from disc to disc. The narrow pore size distribution of cellulose nitrate membranes ensures your retention data is consistent across batches, reducing the inter-sample variability that complicates quantitative particulate and microbiological analyses. Make the decision for consistency and reliability, ensuring that your laboratory maintains its reputation for high-quality, error-free output.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

40.6psi

Color

white

Diameter

25mm

Extractables

low

Filtration Area

4.91cm²

Flow Rate

60ml (Water)

Grid (Yes/No)

No

Material

Cellulose Nitrate (CN)

Maximum Operating Pressure

Holder-dependent

Maximum Operating Temperature (°C)

80°C

Membrane Thickness (µm)

105–140um

Pack Count

100

pH Range

4-9

Pore Size (µm)

0.45

Sterility

Nonsterile

Sterilization

Autoclavable at 121°C for 30 mins

Support Material

None

Wettability

Hydrophilic

• Biochemical Assays - High protein-binding capacity effectively immobilizes macromolecules, supporting lateral flow and diagnostic testing.
• Environmental Compliance - Documented retention characteristics at standard pore ratings support reproducibility in regulated testing programs.
• Colony Enumeration - Gridded surface variants facilitate accurate plate counting of captured microorganisms under standard magnification.
• Gravimetric Particulate Analysis - Dissolves in organic solvents for complete particle recovery and accurate weight-based quantification.
• Microscopy Examination - Particles remain on the membrane surface for direct visual inspection, supporting identification and classification.
• Water Quality Testing - Narrow pore distribution retains indicator organisms consistently for regulatory compliance in environmental laboratories.