ME25/20 STL 0.45um 50mm 400/Pk SKU: 10407324

Mixed Cellulose Ester (MCE) Membrane Filters - 10407324

The Whatman 10407324 combines a controlled pore size distribution with low extractable levels, ensuring the data accuracy that sensitive analytical methods require. Reproducible separation characteristics define the membrane structure, protecting downstream instruments and preserving sample integrity throughout the filtration process. Accordingly, consistent surface properties across the full membrane area support uniform particle capture and reliable results in gravimetric, microscopic, and culture-based analytical workflows.

Material Specifications

• The biologically inert blend of cellulose acetate and cellulose nitrate provides a membrane surface optimized for organism capture and colony enumeration in microbiological assays.
• Hydrophilic by nature, the MCE membrane structure supports high non-specific adsorption for effective organism capture.
• Feature a uniform pore structure that provides consistent flow rates and high throughputs across the full range of standard pore size ratings.
• Mixed Cellulose Ester membranes combine cellulose acetate and cellulose nitrate polymers to deliver a smoother, more flexible membrane surface than pure cellulose nitrate.
• Known for excellent wetting properties and high flow rates in aqueous filtration.

Microbial enumeration methods require complete retention at the rated pore size and a surface that supports colony growth without inhibition, and the membrane specifications above confirm both requirements for your culture-based workflows. MCE's naturally hydrophilic surface allows immediate wetting upon contact with aqueous samples, eliminating the pre-conditioning step and letting you begin filtration as soon as the membrane is seated in your holder. Make the decision for consistency and reliability, ensuring that your laboratory maintains its reputation for high-quality, error-free output.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

40.6psi

Color

black

Diameter

50mm

Extractables

low

Filtration Area

19.63cm²

Flow Rate

60ml (Water)

Grid (Yes/No)

Yes

Material

Mixed Cellulose Esters (MCE)

Maximum Operating Pressure

Holder-dependent

Maximum Operating Temperature (°C)

130°C

Membrane Thickness (µm)

145um

Pack Count

400

pH Range

4-8

Pore Size (µm)

0.45

Sterility

Nonsterile

Sterilization

Autoclavable at 121°C for 30 mins

Support Material

None

Wettability

Hydrophilic

• Black Grid Analysis - Black membrane variants enhance contrast for light-colored colonies, reducing eye strain during manual plate counting.
• Aqueous Filtration - Biologically inert composition provides high flow rates and consistent reliability for routine laboratory fluid processing.
• Microscopy mounting - Can be made transparent with immersion oil, allowing for direct light microscopy of retained particles on the surface.
• Aerosol Analysis - Dissolves completely in acid digestion, allowing for the total chemical analysis of captured airborne metal particulates.
• Biochemical Assays - High electrostatic properties effectively bind proteins and macromolecules, supporting various diagnostic and research assays.
• Chemotaxis Assays - Acts as a barrier in Boyden chambers, allowing cells to migrate through defined pores towards a chemoattractant source.