Whatman
AE100 12 um 47mm 100/Pk SKU: 10400012

Cellulose Nitrate (CN) Membrane Filters - 10400012

The 10400012 combines a controlled pore size distribution with low extractable levels, ensuring the data accuracy that sensitive analytical methods require. Precision membrane geometry balances retention sharpness with sustainable flow rates, minimizing the pressure buildup that leads to premature filter replacement. From raw polymer through final inspection, the manufacturing process is designed to ensure that every membrane disc meets the performance specifications of its grade designation.

Material Specifications

• Thermal stability of the construction materials allows safe operation at temperatures up to 80°C without degradation of membrane or housing performance.
• High protein binding capacity makes cellulose nitrate the preferred membrane for Western blotting, dot blotting, and nucleic acid immobilization techniques that rely on efficient surface adsorption.
• Dissolves completely in specific organic solvents, enabling full recovery of captured particles from the membrane surface for downstream gravimetric, chemical, or spectroscopic analysis.
• Engineered to withstand operating pressures reaching > 10 psi, the construction prevents housing deformation that could compromise membrane retention.
• Packaged without sterilization for applications where downstream sterility is achieved by other means or is not a process requirement.

If your protocol requires direct on-membrane analysis by microscopy or culture, the surface characteristics and dimensional tolerances described above give you a substrate that performs consistently from disc to disc. Because cellulose nitrate dissolves completely in organic solvents like acetone and amyl acetate, you can recover every captured particle from the membrane surface for downstream gravimetric, spectroscopic, or chemical analysis. If your laboratory requires volume pricing, custom packaging configurations, or scheduled delivery programs, the Tisch Scientific sales team can structure a supply arrangement matched to your consumption rate.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Diameter

47mm

Pore Size (µm)

12

Sterility

Nonsterile

Wetability

Hydrophilic

• Vacuum Clarification - Utilized in Buchner funnels to rapidly remove particulates from large-volume batches of non-critical laboratory buffers.
• Quality Control Testing - Serves as a standard check filter for routine batch purity tests, verifying the absence of visible physical contaminants.
• Gravimetric Analysis - Captures total suspended solids from liquid samples on a weighed disc, ensuring accurate mass measurements for reporting.
• Stack Pre-filtration - Acts as a spacer or depth layer in multi-tier filtration stacks to extend the life of the final downstream membrane.
• Air Particulate Sampling - Traps airborne dust and pollen on a positioned open-face filter disc for subsequent microscopic or chemical analysis.
• Radiometric Counting - Collects radioactive precipitates for scintillation counting, providing a consistent geometry for accurate data measurement.