AE99 8.0um 25mm 100/Pk SKU: 10400106

Cellulose Nitrate (CN) Membrane Filters - 10400106

The Whatman 10400106 combines a controlled pore size distribution with low extractable levels, ensuring the data accuracy that sensitive analytical methods require. In addition, verified 8.0 pore distribution across the Hydrophilic membrane surface guarantees batch-to-batch consistency in particle capture and analyte passage. In practice, consistent surface properties across the full membrane area support uniform particle capture and reliable results in gravimetric, microscopic, and culture-based analytical workflows.

Material Specifications

• Autoclavable at 121°C without loss of integrity, cellulose nitrate membranes maintain their rated pore structure and dimensional stability through repeated sterilization cycles.
• Economical bulk packaging supports laboratories with high consumable throughput where individual sterile wrapping is unnecessary.
• High protein binding capacity makes cellulose nitrate the preferred membrane for Western blotting, dot blotting, and nucleic acid immobilization techniques that rely on efficient surface adsorption.
• Cellulose nitrate membranes feature a narrow pore size distribution and low extractable levels, providing the controlled retention characteristics required for gravimetric and particulate analysis.
• Dissolves completely in specific organic solvents, enabling full recovery of captured particles from the membrane surface for downstream gravimetric, chemical, or spectroscopic analysis.

Reporting filtration parameters alongside your analytical results demands documented pore size, material chemistry, and lot traceability — the membrane specifications above provide the specificity that reviewers and auditors expect. To meet this need, the narrow pore size distribution of cellulose nitrate membranes ensures your retention data is consistent across batches, reducing the inter-sample variability that complicates quantitative particulate and microbiological analyses. Secure your research outcomes by partnering with a filtration solution that understands your need for speed, accuracy, and reproducibility.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

N/A

Color

white

Diameter

25mm

Extractables

low

Filtration Area

4.91cm²

Flow Rate

400ml (Water)

Grid (Yes/No)

No

Material

Cellulose Nitrate (CN)

Maximum Operating Pressure

Holder-dependent

Maximum Operating Temperature (°C)

80°C

Membrane Thickness (µm)

105–140um

Pack Count

100

pH Range

4-8

Pore Size (µm)

8

Sterility

Nonsterile

Sterilization

Autoclavable at 121°C for 30 mins

Support Material

None

Wettability

Hydrophilic

• Environmental Compliance - Documented retention characteristics at standard pore ratings support reproducibility in regulated testing programs.
• Gravimetric Particulate Analysis - Dissolves in organic solvents for complete particle recovery and accurate weight-based quantification.
• Biochemical Assays - High protein-binding capacity effectively immobilizes macromolecules, supporting lateral flow and diagnostic testing.
• Microscopy Examination - Particles remain on the membrane surface for direct visual inspection, supporting identification and classification.
• Colony Enumeration - Gridded surface variants facilitate accurate plate counting of captured microorganisms under standard magnification.
• Bioburden Monitoring - Surface retention keeps captured organisms accessible for direct incubation and enumeration without transfer steps.