AE98 5.0um 25mm 100/Pk SKU: 10400206

Cellulose Nitrate (CN) Membrane Filters - 10400206

Designed to deliver reproducibility and confidence across critical sample types, the Whatman 10400206 provides the filtration consistency that method validation demands. At its core, precision-manufactured Hydrophilic membrane with a verified 5.0 pore structure delivers consistent retention across the full operating range. By design, each membrane disc reflects a manufacturing standard where retention characteristics, physical integrity, and dimensional accuracy are verified before release.

Material Specifications

• Naturally hydrophilic and compatible with aqueous solutions, cellulose nitrate delivers high flow rates and rapid wet-out without requiring pre-conditioning or surfactant treatment.
• Surface retention characteristics allow captured particles and microorganisms to remain on the membrane face, supporting direct microscopic examination and physical recovery of collected material.
• Bulk format reduces per-unit packaging waste and cost, appropriate for routine particulate removal and clarification where aseptic handling is not specified.
• High protein binding capacity makes cellulose nitrate the preferred membrane for Western blotting, dot blotting, and nucleic acid immobilization techniques that rely on efficient surface adsorption.
• Autoclavable at 121°C without loss of integrity, cellulose nitrate membranes maintain their rated pore structure and dimensional stability through repeated sterilization cycles.

Maintaining accreditation under ISO 17025 or similar quality frameworks requires documented traceability at every step — the specification compliance of each membrane lot supports your ongoing audit readiness. Specifically, cellulose nitrate's dimensional stability through autoclaving at 121°C means your membranes maintain their rated pore structure and physical dimensions from pre-sterilization through final analysis, supporting method reproducibility. Take control of your sample preparation variables and ensure that your data reflects the true nature of your work, rather than the quality of your consumables.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

6-8psi

Color

white

Diameter

25mm

Extractables

low

Filtration Area

4.91cm²

Flow Rate

350ml (Water)

Grid (Yes/No)

No

Material

Cellulose Nitrate (CN)

Maximum Operating Pressure

Holder-dependent

Maximum Operating Temperature (°C)

80°C

Membrane Thickness (µm)

105–140um

Pack Count

100

pH Range

4-8

Pore Size (µm)

5

Sterility

Nonsterile

Sterilization

Autoclavable at 121°C for 30 mins

Support Material

None

Wettability

Hydrophilic

• Environmental Compliance - Documented retention characteristics at standard pore ratings support reproducibility in regulated testing programs.
• Bioburden Monitoring - Surface retention keeps captured organisms accessible for direct incubation and enumeration without transfer steps.
• Biochemical Assays - High protein-binding capacity effectively immobilizes macromolecules, supporting lateral flow and diagnostic testing.
• Water Quality Testing - Narrow pore distribution retains indicator organisms consistently for regulatory compliance in environmental laboratories.
• Colony Enumeration - Gridded surface variants facilitate accurate plate counting of captured microorganisms under standard magnification.
• Routine Sample Prep - Uniform microporous matrix provides consistent flow rates and reliable particle retention for general aqueous solutions.