Whatman
AE98 5.0um 47mm 100/Pk SKU: 10400212

Cellulose Nitrate (CN) Membrane Filters - 10400212

The 10400212 combines a controlled pore size distribution with low extractable levels, ensuring the data accuracy that sensitive analytical methods require. Verified 5.0 pore distribution across the Hydrophilic membrane surface guarantees batch-to-batch consistency in particle capture and analyte passage. Controlled production conditions produce membranes with documented pore size distribution and thickness tolerances, supporting method reproducibility across multiple filter lots.

Material Specifications

• Thermal stability of the construction materials allows safe operation at temperatures up to 80°C without degradation of membrane or housing performance.
• Autoclavable at 121°C without loss of integrity, cellulose nitrate membranes maintain their rated pore structure and dimensional stability through repeated sterilization cycles.
• Packaged without sterilization for applications where downstream sterility is achieved by other means or is not a process requirement.
• Engineered to withstand operating pressures reaching > 10 psi, the construction prevents housing deformation that could compromise membrane retention.
• High protein binding capacity makes cellulose nitrate the preferred membrane for Western blotting, dot blotting, and nucleic acid immobilization techniques that rely on efficient surface adsorption.

Reporting filtration parameters alongside your analytical results demands documented pore size, material chemistry, and lot traceability — the membrane specifications above provide the specificity that reviewers and auditors expect. Surface retention on cellulose nitrate keeps captured particles and microorganisms on the membrane face where they remain accessible for direct microscopic examination, colony counting, and physical recovery without sectioning or dissolution. For questions about chemical compatibility, operating limits, or equivalent product cross-references, Tisch Scientific's filtration specialists can provide the technical detail your method documentation requires.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Diameter

47mm

Pore Size (µm)

5

Sterility

Nonsterile

Wetability

Hydrophilic

• Microscopy mounting - Retains microorganisms on a flat surface, allowing for direct staining and enumeration under a high-power microscope.
• Gravimetric Analysis - Captures total suspended solids from liquid samples on a weighed disc, ensuring accurate mass measurements for reporting.
• Stack Pre-filtration - Acts as a spacer or depth layer in multi-tier filtration stacks to extend the life of the final downstream membrane.
• Quality Control Testing - Serves as a standard check filter for routine batch purity tests, verifying the absence of visible physical contaminants.
• Vacuum Clarification - Utilized in Buchner funnels to rapidly remove particulates from large-volume batches of non-critical laboratory buffers.
• Air Particulate Sampling - Traps airborne dust and pollen on a positioned open-face filter disc for subsequent microscopic or chemical analysis.