Sterile MicroPlus -21 STL Membrane, w/3.1mm Black Grid for Membrane-Butler 0.45um 50mm Circle 400pcs SKU: 10407114

Cellulose Nitrate (CN) Membrane Filters - 10407114

Designed to deliver reproducibility and confidence across critical sample types, the Whatman 10407114 provides the filtration consistency that method validation demands. Supporting this capability, reproducible separation characteristics define the membrane structure, protecting downstream instruments and preserving sample integrity throughout the filtration process. Precision manufacturing ensures that each membrane disc delivers the rated retention, flow rate, and surface consistency that analytical and microbiological methods require.

Material Specifications

• Autoclavable at 121°C without loss of integrity, cellulose nitrate membranes maintain their rated pore structure and dimensional stability through repeated sterilization cycles.
• Dissolves completely in specific organic solvents, enabling full recovery of captured particles from the membrane surface for downstream gravimetric, chemical, or spectroscopic analysis.
• Naturally hydrophilic and compatible with aqueous solutions, cellulose nitrate delivers high flow rates and rapid wet-out without requiring pre-conditioning or surfactant treatment.
• Cellulose nitrate membranes feature a narrow pore size distribution and low extractable levels, providing the controlled retention characteristics required for gravimetric and particulate analysis.
• High protein binding capacity makes cellulose nitrate the preferred membrane for Western blotting, dot blotting, and nucleic acid immobilization techniques that rely on efficient surface adsorption.

If your protocol requires direct on-membrane analysis by microscopy or culture, the surface characteristics and dimensional tolerances described above give you a substrate that performs consistently from disc to disc. To support this, the narrow pore size distribution of cellulose nitrate membranes ensures your retention data is consistent across batches, reducing the inter-sample variability that complicates quantitative particulate and microbiological analyses. Elevate the quality of your analytical data today by standardizing on a filtration product built to meet the exact specifications of your applications.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

40.6psi

Color

white

Diameter

50mm

Extractables

low

Filtration Area

19.63cm²

Flow Rate

60ml (Water)

Grid (Yes/No)

Yes

Material

Cellulose Nitrate (CN)

Maximum Operating Pressure

Holder-dependent

Maximum Operating Temperature (°C)

121°C

Membrane Thickness (µm)

145um

Pack Count

400

pH Range

4-8

Pore Size (µm)

0.45

Sterility

Sterile

Sterilization

Supplied Sterile (Single Use)

Support Material

None

Wettability

Hydrophilic

• Gravimetric Particulate Analysis - Dissolves in organic solvents for complete particle recovery and accurate weight-based quantification.
• Biochemical Assays - High protein-binding capacity effectively immobilizes macromolecules, supporting lateral flow and diagnostic testing.
• Bioburden Monitoring - Surface retention keeps captured organisms accessible for direct incubation and enumeration without transfer steps.
• Microscopy Examination - Particles remain on the membrane surface for direct visual inspection, supporting identification and classification.
• Routine Sample Prep - Uniform microporous matrix provides consistent flow rates and reliable particle retention for general aqueous solutions.
• Colony Enumeration - Gridded surface variants facilitate accurate plate counting of captured microorganisms under standard magnification.