NYTRAN N2 300mm x 3m 1/Pk SKU: 10416096

Nytran Nylon Blotting Membranes - 10416096

Demanding nucleic acid applications require a membrane cast with uniform pore distribution on both sides of a reinforced support matrix to eliminate curling and ensure reproducible transfers. Dual-cast manufacturing deposits nylon uniformly on both sides of an integrated support matrix, creating a symmetrical membrane that lies flat during transfer without the curling that disrupts capillary blotting setups. Proven lot-to-lot reproducibility reduces method optimization time and supports standardized blotting protocols across laboratory teams.

Membrane Specifications

• Chemical resistance to standard hybridization buffers, SSC solutions, and stripping reagents ensures reliable membrane performance across diverse blotting protocols.
• Moderate positive surface charge creates ionic interactions with negatively charged nucleic acid backbones for reliable target immobilization under standard transfer conditions.
• Manufactured with precise control over charge density and pore distribution, Nytran nylon membranes deliver consistent hybridization signal intensity across the entire membrane surface for reproducible blot-to-blot results.
• Selected 0.2μm pore rating optimizes retention characteristics for your target molecular weight range during electrophoretic or capillary transfer.
• High mechanical strength withstands multiple rounds of probe stripping and rehybridization without membrane degradation or loss of bound target signal.

Whether your experiments involve routine Western blots or multi-probe hybridization studies, the binding consistency and low background documented above protect the clarity of every result you publish. Controlled positive charge density balances maximum nucleic acid capture with the low background properties essential for detecting low-copy-number gene targets in complex genomic samples. If your laboratory requires volume pricing, custom packaging configurations, or scheduled delivery programs, the Tisch Scientific sales team can structure a supply arrangement matched to your consumption rate.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Dimensions

300mm x 3m

Material

Nylon

Sterility

Nonsterile

Wettability

Hydrophilic

• Nucleic Acid Library Screening — High-throughput identification of target clones from genomic and cDNA libraries using labeled probes.
• Northern Blotting — Transfer and probe hybridization of total or mRNA for tissue-specific gene expression profiling and transcript characterization.
• Colony and Plaque Lifts — High-density library screening via in situ transfer and hybridization of bacterial colonies or bacteriophage plaques.
• UV Crosslinking Immobilization — Permanent covalent fixation of transferred nucleic acids for long-term storage and repeated hybridization analysis.
• Multiple Probe Rehybridization — Sequential stripping and reprobing of a single membrane with different labeled probes for multi-target analysis.
• Southern Blotting — Alkaline capillary transfer and hybridization of genomic DNA restriction fragments for gene detection and structural analysis.