NYTRAN N2 300mm x 3m 1/Pk
Whatman SKU: 10416096

Nylon Blotting Membranes, 0.2 µm, 30 mm x 3 m, 1/roll - 10416096

Whatman holds surface and binding characteristics steady from lot to lot, giving laboratories the reproducibility that repeated transfer and detection work demands. Charged nylon transfer membranes capture transferred DNA and RNA for hybridization. Charged nylon supports radioactive and chemiluminescent hybridization while permanently fixing nucleic acids by UV or baking and supports many hybridization cycles. Where membranes face repeated probing, charged nylon carries the blot through multiple hybridization rounds.

Key Characteristics

• Compatible with alkaline transfer and isotopic or non-isotopic detection
• Optimized for Southern, Northern, and hybridization-based screening
• High nucleic-acid binding captures transferred DNA and RNA
• Positively charged surface anchors nucleic acids electrostatically
• The 0.2 micron pore optimally retains oligonucleotides and small DNA fragments during transfer and immobilization

Reliable nucleic-acid capture across the membrane keeps your hybridization signal clean and repeatable. Dependable nucleic-acid immobilization holds your transferred DNA and RNA through hybridization through baking or UV crosslinking for permanent fixation. Ask our friendly customer service team to confirm the format and binding capacity your blotting protocol needs.

Dimensions

300mm x 3m

Material

Nylon

Sterility

Nonsterile

Wettability

Hydrophilic

• UV Crosslink Immobilization - Permanently fixes transferred nucleic acids for long-term storage and repeated probe hybridization.
• Southern Blotting - Captures restriction-digested DNA for hybridization-based gene detection and structural mapping analysis.
• Dot and Slot Blotting - Anchors directly applied nucleic acids for rapid sequence-specific screening and quantitation analysis.
• Multiple Reprobing - Withstands repeated stripping and reprobing for multi-target hybridization analysis on a single membrane.
• Colony and Plaque Lifts - Transfers and immobilizes nucleic acids for hybridization screening of genomic and cDNA libraries.
• Northern Blotting - Immobilizes size-fractionated RNA for gene expression profiling and transcript size characterization.