NYTRAN N45 300mm x 3m 1/Pk
Whatman SKU: 10416196

Nylon Blotting Membranes, 0.45 µm, 30 mm x 3 m, 1/roll - 10416196

Whatman's brand recognition and documented performance history support specification of its membranes in standardized blotting and transfer protocols. Charged nylon blotting membranes immobilize nucleic acids after gel transfer. Positively charged nylon holds nucleic acids through hybridization while locking DNA and RNA in place for repeated probing and withstands repeated stripping and reprobing. Where membranes face repeated probing, charged nylon carries the blot through multiple hybridization rounds.

Key Characteristics

• Compatible with alkaline transfer and isotopic or non-isotopic detection
• The 0.45 micron pore suits transfer of larger DNA and RNA fragments onto the charged membrane
• High nucleic-acid binding captures transferred DNA and RNA
• Optimized for Southern, Northern, and hybridization-based screening
• Positively charged surface anchors nucleic acids electrostatically

Holding transferred DNA or RNA firmly through hybridization keeps your Southern and Northern blots sensitive. Durable nucleic-acid capture supports repeated stripping and reprobing across Southern, Northern, and colony or plaque screening. Connect with our customer support technicians to choose between PVDF, nitrocellulose, and nylon for your blots.

Dimensions

300mm x 3m

Material

Nylon

Sterility

Nonsterile

Wettability

Hydrophilic

• Multiple Reprobing - Withstands repeated stripping and reprobing for multi-target hybridization analysis on a single membrane.
• Southern Blotting - Captures restriction-digested DNA for hybridization-based gene detection and structural mapping analysis.
• UV Crosslink Immobilization - Permanently fixes transferred nucleic acids for long-term storage and repeated probe hybridization.
• Northern Blotting - Immobilizes size-fractionated RNA for gene expression profiling and transcript size characterization.
• Colony and Plaque Lifts - Transfers and immobilizes nucleic acids for hybridization screening of genomic and cDNA libraries.
• Dot and Slot Blotting - Anchors directly applied nucleic acids for rapid sequence-specific screening and quantitation analysis.