NYTRAN N45 300mm x 3m 1/Pk SKU: 10416196

Nytran Nylon Blotting Membranes - 10416196

Demanding nucleic acid applications require a membrane cast with uniform pore distribution on both sides of a reinforced support matrix to eliminate curling and ensure reproducible transfers. Broad chemical compatibility with standard hybridization buffers, SSC and alkaline transfer solutions, and common stripping reagents ensures reliable performance across diverse blotting protocols. Broad detection method compatibility provides the analytical flexibility to adapt workflows as experimental requirements evolve.

Membrane Specifications

• Manufactured with precise control over charge density and pore distribution, Nytran nylon membranes deliver consistent hybridization signal intensity across the entire membrane surface for reproducible blot-to-blot results.
• Pore size of 0.45μm balances efficient target capture with controlled transfer speed to minimize blow-through of lower molecular weight biomolecules.
• Binding capacity exceeding 400 μg/cm² provides abundant surface sites for efficient capture of DNA and RNA targets during transfer.
• High mechanical strength withstands multiple rounds of probe stripping and rehybridization without membrane degradation or loss of bound target signal.
• High mechanical strength and chemical resistance allow Nytran nylon membranes to maintain structural integrity through alkaline transfers, high-temperature hybridizations, and repeated stripping cycles without signal degradation.

If your laboratory runs standardized blotting protocols across multiple analysts or shifts, the dimensional precision and binding uniformity of this membrane reduce the operator-to-operator variability that can compromise your data quality. Controlled positive charge density balances maximum nucleic acid capture with the low background properties essential for detecting low-copy-number gene targets in complex genomic samples. Complete product specifications, certificates of analysis, and lot-specific documentation to support your quality system requirements are available through Tisch Scientific upon request.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Dimensions

300mm x 3m

Material

Nylon

Sterility

Nonsterile

Wettability

Hydrophilic

• Dot and Slot Blotting — Rapid nucleic acid quantitation and sequence-specific screening by direct sample application to charged nylon.
• UV Crosslinking Immobilization — Permanent covalent fixation of transferred nucleic acids for long-term storage and repeated hybridization analysis.
• Northern Blotting — Transfer and probe hybridization of total or mRNA for tissue-specific gene expression profiling and transcript characterization.
• Colony and Plaque Lifts — High-density library screening via in situ transfer and hybridization of bacterial colonies or bacteriophage plaques.
• Nucleic Acid Library Screening — High-throughput identification of target clones from genomic and cDNA libraries using labeled probes.
• Restriction Fragment Length Analysis — Detection of polymorphic DNA fragments by Southern hybridization for genotyping and linkage mapping studies.