NYTRAN SPC 110x140mm 10/Pk SKU: 10416230

Nytran Nylon Blotting Membranes - 10416230

From routine DNA restriction analysis to complex multi-probe rehybridization studies, reliable results begin with a nylon membrane built for both sensitivity and mechanical resilience. Broad chemical compatibility with standard hybridization buffers, SSC and alkaline transfer solutions, and common stripping reagents ensures reliable performance across diverse blotting protocols. Reliable transfer performance delivers the binding efficiency and signal clarity needed for confident data interpretation in quantitative applications.

Membrane Specifications

• High mechanical strength and chemical resistance allow Nytran nylon membranes to maintain structural integrity through alkaline transfers, high-temperature hybridizations, and repeated stripping cycles without signal degradation.
• Nytran nylon blotting membranes are cast uniformly on both sides of a reinforced support matrix, providing the symmetrical structure that prevents curling and ensures flat, even contact during capillary and electrophoretic transfer.
• Positively charged nylon construction gives Nytran membranes the ionic binding strength to immobilize nucleic acids permanently under alkaline transfer conditions, eliminating post-transfer crosslinking steps that add time and variability to standard protocols.
• Engineered for Southern, Northern, colony lift, and dot blot applications, Nytran membranes support both radioactive and nonradioactive detection systems with the sensitivity needed for low-copy-number target sequences.
• Manufactured with precise control over charge density and pore distribution, Nytran nylon membranes deliver consistent hybridization signal intensity across the entire membrane surface for reproducible blot-to-blot results.

For your applications where target retention and background noise directly determine whether low-abundance bands are detectable, the surface chemistry and pore structure of this membrane give you the sensitivity margin to resolve what matters. Controlled positive charge density balances maximum nucleic acid capture with the low background properties essential for detecting low-copy-number gene targets in complex genomic samples. Complete product specifications, certificates of analysis, and lot-specific documentation to support your quality system requirements are available through Tisch Scientific upon request.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Dimensions

110mm x 140mm

Material

Nylon

Sterility

Nonsterile

Wettability

Hydrophilic

• Colony and Plaque Lifts — High-density library screening via in situ transfer and hybridization of bacterial colonies or bacteriophage plaques.
• Northern Blotting — Transfer and probe hybridization of total or mRNA for tissue-specific gene expression profiling and transcript characterization.
• Dot and Slot Blotting — Rapid nucleic acid quantitation and sequence-specific screening by direct sample application to charged nylon.
• UV Crosslinking Immobilization — Permanent covalent fixation of transferred nucleic acids for long-term storage and repeated hybridization analysis.
• Southern Blotting — Alkaline capillary transfer and hybridization of genomic DNA restriction fragments for gene detection and structural analysis.
• Nucleic Acid Library Screening — High-throughput identification of target clones from genomic and cDNA libraries using labeled probes.