NYTRAN SPC 110x140mm 10/Pk
Whatman SKU: 10416230

Nylon Blotting Membranes, 0.45 µm, 110 mm x 140 mm, 10/pk - 10416230

Whatman's brand recognition and documented performance history support specification of its membranes in standardized blotting and transfer protocols. Supercharged high-density nylon captures transferred DNA and RNA at very high capacity. UV crosslinking or baking permanently fixes nucleic acids to the membrane while enduring successive strip-and-reprobe rounds and anchors DNA and RNA through a very high positive charge. For permanent immobilization at maximal capacity, UV-crosslinked supercharged nylon fixes DNA and RNA for repeated hybridization.

Key Characteristics

• UV crosslinking or baking permanently immobilizes nucleic acids on the membrane
• Compatible with alkaline transfer and isotopic or non-isotopic detection
• The 0.45 micron pore suits transfer of larger DNA and RNA fragments onto the charged membrane
• Extremely low background enables high-sensitivity low-copy-number detection
• Supercharged positive surface anchors nucleic acids at very high density

Reliable nucleic-acid capture across the membrane keeps your hybridization signal clean and repeatable. Dependable nucleic-acid immobilization holds your transferred DNA and RNA through hybridization so probes reach their targets at high sensitivity. Ask our friendly customer service team to confirm the format and binding capacity your blotting protocol needs.

Dimensions

110mm x 140mm

Material

Nylon

Sterility

Nonsterile

Wettability

Hydrophilic

• Low-Copy Detection - Captures scarce nucleic-acid targets at very high density for sensitive Southern and Northern hybridization.
• Multiple Reprobing - Withstands repeated stripping and reprobing across many high-sensitivity hybridization cycles per membrane.
• Southern Blotting - Immobilizes restriction-digested DNA on a supercharged surface for sensitive hybridization-based gene detection.
• UV Crosslink Immobilization - Permanently fixes transferred nucleic acids for long-term storage and repeated high-sensitivity reprobing.
• Colony and Plaque Lifts - Captures nucleic acids at high density for hybridization screening of genomic and cDNA libraries.
• Northern Blotting - Anchors size-fractionated RNA at very high capacity for low-abundance transcript expression analysis.