NYTRAN SPC 200mm x 3m 1/Pk
Whatman SKU: 10416294

Nylon Blotting Membranes, 0.45 µm, 20 mm x 3 m, 1/roll - 10416294

Whatman's expertise in membrane casting guides the surface chemistry and quality control behind reproducible capture of transferred molecules. A very high positive surface charge anchors transferred nucleic acids across the membrane. Covalent crosslinking locks DNA and RNA in place for repeated probing while surfacing faint low-abundance hybridization signals and holds nucleic acids through its dense positive charge. For permanent immobilization at maximal capacity, UV-crosslinked supercharged nylon fixes DNA and RNA for repeated hybridization.

Key Characteristics

• Very high nucleic-acid binding above 600 ug/cm2 captures transferred DNA and RNA
• UV crosslinking or baking permanently immobilizes nucleic acids on the membrane
• Extremely low background enables high-sensitivity low-copy-number detection
• Durable construction withstands repeated stripping and reprobing cycles
• Supercharged positive surface anchors nucleic acids at very high density

Capturing nucleic acids and crosslinking them permanently sets up the repeat probing your analysis needs. Dependable nucleic-acid immobilization holds your transferred DNA and RNA through hybridization through baking or UV crosslinking for permanent fixation. Connect with our customer support technicians to choose between PVDF, nitrocellulose, and nylon for your blots.

Dimensions

200mm x 3m

Material

Nylon

Sterility

Nonsterile

Wettability

Hydrophilic

• Multiple Reprobing - Withstands repeated stripping and reprobing across many high-sensitivity hybridization cycles per membrane.
• Northern Blotting - Anchors size-fractionated RNA at very high capacity for low-abundance transcript expression analysis.
• Southern Blotting - Immobilizes restriction-digested DNA on a supercharged surface for sensitive hybridization-based gene detection.
• UV Crosslink Immobilization - Permanently fixes transferred nucleic acids for long-term storage and repeated high-sensitivity reprobing.
• Colony and Plaque Lifts - Captures nucleic acids at high density for hybridization screening of genomic and cDNA libraries.
• Low-Copy Detection - Captures scarce nucleic-acid targets at very high density for sensitive Southern and Northern hybridization.