Whatman
NYTRAN SPC 200mm x 3m 1/Pk SKU: 10416294

Nytran Nylon Blotting Membranes - 10416294

Southern and Northern blotting workflows produce the most reliable data when built on a positively charged nylon surface engineered for permanent nucleic acid immobilization under alkaline transfer conditions. Broad chemical compatibility with standard hybridization buffers, SSC and alkaline transfer solutions, and common stripping reagents ensures reliable performance across diverse blotting protocols. Consistent binding performance and low background noise support reliable detection across experiment types and throughput levels.

Membrane Specifications

• Positively charged nylon construction gives Nytran membranes the ionic binding strength to immobilize nucleic acids permanently under alkaline transfer conditions, eliminating post-transfer crosslinking steps that add time and variability to standard protocols.
• Very uniform pore size and pore distribution across the membrane surface ensures consistent hybridization signal intensity for reproducible blot-to-blot comparisons.
• Maximum operating temperature of 135°C supports autoclave sterilization on a liquid cool cycle for applications requiring aseptic membrane preparation.
• Dual-cast nylon construction on a reinforced support matrix provides the symmetrical structure that prevents membrane curling during capillary transfer setup.
• Pore size of 0.45μm balances efficient target capture with controlled transfer speed to minimize blow-through of lower molecular weight biomolecules.

When your research timeline depends on getting defensible blotting data without repeated optimization cycles, the transfer efficiency and signal clarity built into this membrane help you move from experiment to answer with confidence. Symmetrical dual-cast construction prevents membrane curling and delivers uniform signal intensity across the blot surface, improving the accuracy of densitometric quantitation and side-by-side comparisons. Sample quantities for method validation and compatibility testing are available through Tisch Scientific — request a sample to verify performance in your specific workflow before committing to a production volume.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Dimensions

200mm x 3m

Material

Nylon

Sterility

Nonsterile

Wettability

Hydrophilic

• Multiple Probe Rehybridization — Sequential stripping and reprobing of a single membrane with different labeled probes for multi-target analysis.
• Nucleic Acid Library Screening — High-throughput identification of target clones from genomic and cDNA libraries using labeled probes.
• Dot and Slot Blotting — Rapid nucleic acid quantitation and sequence-specific screening by direct sample application to charged nylon.
• Restriction Fragment Length Analysis — Detection of polymorphic DNA fragments by Southern hybridization for genotyping and linkage mapping studies.
• Southern Blotting — Alkaline capillary transfer and hybridization of genomic DNA restriction fragments for gene detection and structural analysis.
• Colony and Plaque Lifts — High-density library screening via in situ transfer and hybridization of bacterial colonies or bacteriophage plaques.