NYTRAN SPC 300mm x 3m 1/Pk
Whatman SKU: 10416296

Nylon Blotting Membranes, 0.45 µm, 30 mm x 3 m, 1/roll - 10416296

Whatman's brand recognition and documented performance history support specification of its membranes in standardized blotting and transfer protocols. Supercharged high-density nylon captures transferred DNA and RNA at very high capacity. Charged Nytran SPC nylon immobilizes nucleic acids at very high density and permanently fixes nucleic acids by UV crosslinking or baking. When low-copy-number targets must be seen, supercharged nylon captures nucleic acids at very high density for sensitive hybridization.

Key Characteristics

• Compatible with alkaline transfer and isotopic or non-isotopic detection
• The 0.45 micron pore suits transfer of larger DNA and RNA fragments onto the charged membrane
• Very high nucleic-acid binding above 600 ug/cm2 captures transferred DNA and RNA
• UV crosslinking or baking permanently immobilizes nucleic acids on the membrane
• Extremely low background enables high-sensitivity low-copy-number detection

Holding transferred DNA or RNA firmly through hybridization keeps your Southern and Northern blots sensitive. Dependable nucleic-acid immobilization holds your transferred DNA and RNA through hybridization across capillary and alkaline transfer. Send your target protein or nucleic acid details to our technical service team for a membrane recommendation.

Dimensions

300mm x 3m

Material

Nylon

Sterility

Nonsterile

Wettability

Hydrophilic

• Northern Blotting - Anchors size-fractionated RNA at very high capacity for low-abundance transcript expression analysis.
• Colony and Plaque Lifts - Captures nucleic acids at high density for hybridization screening of genomic and cDNA libraries.
• Low-Copy Detection - Captures scarce nucleic-acid targets at very high density for sensitive Southern and Northern hybridization.
• UV Crosslink Immobilization - Permanently fixes transferred nucleic acids for long-term storage and repeated high-sensitivity reprobing.
• Multiple Reprobing - Withstands repeated stripping and reprobing across many high-sensitivity hybridization cycles per membrane.
• Southern Blotting - Immobilizes restriction-digested DNA on a supercharged surface for sensitive hybridization-based gene detection.