NYTRAN SPC 300mm x 3m 1/Pk SKU: 10416296

Nytran Nylon Blotting Membranes - 10416296

High-throughput genomic screening and gene expression analysis depend on nylon membranes that deliver consistent binding performance across every region of the membrane surface. Reinforced nylon construction provides the tear resistance and handling durability needed for multiple probe stripping and rehybridization cycles without membrane degradation or signal loss. Broad detection method compatibility provides the analytical flexibility to adapt workflows as experimental requirements evolve.

Membrane Specifications

• Alkaline transfer compatibility enables permanent nucleic acid immobilization without post-transfer UV crosslinking for streamlined Southern and Northern blot workflows.
• Nytran membranes combine high nucleic acid binding capacity with mechanical durability that withstands multiple rounds of stripping and reprobing, making them the preferred substrate for multi-probe hybridization experiments.
• Positively charged nylon construction gives Nytran membranes the ionic binding strength to immobilize nucleic acids permanently under alkaline transfer conditions, eliminating post-transfer crosslinking steps that add time and variability to standard protocols.
• Chemical resistance to standard hybridization buffers, SSC solutions, and stripping reagents ensures reliable membrane performance across diverse blotting protocols.
• High mechanical strength withstands multiple rounds of probe stripping and rehybridization without membrane degradation or loss of bound target signal.

Whether your experiments involve routine Western blots or multi-probe hybridization studies, the binding consistency and low background documented above protect the clarity of every result you publish. Controlled positive charge density balances maximum nucleic acid capture with the low background properties essential for detecting low-copy-number gene targets in complex genomic samples. If you need help matching a membrane chemistry and pore size to your sample matrix, Tisch Scientific's technical staff can walk you through the selection process and provide application-specific recommendations.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Dimensions

300mm x 3m

Material

Nylon

Sterility

Nonsterile

Wettability

Hydrophilic

• Colony and Plaque Lifts — High-density library screening via in situ transfer and hybridization of bacterial colonies or bacteriophage plaques.
• Northern Blotting — Transfer and probe hybridization of total or mRNA for tissue-specific gene expression profiling and transcript characterization.
• UV Crosslinking Immobilization — Permanent covalent fixation of transferred nucleic acids for long-term storage and repeated hybridization analysis.
• Dot and Slot Blotting — Rapid nucleic acid quantitation and sequence-specific screening by direct sample application to charged nylon.
• Southern Blotting — Alkaline capillary transfer and hybridization of genomic DNA restriction fragments for gene detection and structural analysis.
• Multiple Probe Rehybridization — Sequential stripping and reprobing of a single membrane with different labeled probes for multi-target analysis.