TURBOBL REFl NY-SPC 110x140mm
Whatman SKU: 10416306

Nylon Blotting Membranes, 0.45 µm, 11 cm x 14 cm, 1/pk - 10416306

Whatman produces its membranes to high purity, supporting low background and clean signal across chemiluminescent, colorimetric, fluorescent, and isotopic detection. High-binding supercharged nylon holds DNA and RNA through repeated hybridization rounds. High-density binding surfaces faint low-abundance hybridization signals and secures the transferred targets covalently. When low-copy-number targets must be seen, supercharged nylon captures nucleic acids at very high density for sensitive hybridization.

Key Characteristics

• Compatible with alkaline transfer and isotopic or non-isotopic detection
• Extremely low background enables high-sensitivity low-copy-number detection
• Very high nucleic-acid binding above 600 ug/cm2 captures transferred DNA and RNA
• Durable construction withstands repeated stripping and reprobing cycles
• The 0.45 micron pore suits transfer of larger DNA and RNA fragments onto the charged membrane

Holding transferred DNA or RNA firmly through hybridization keeps your Southern and Northern blots sensitive. Dependable nucleic-acid immobilization holds your transferred DNA and RNA through hybridization across capillary and alkaline transfer. Contact Tisch Scientific for help selecting the right blotting membrane for your transfer and detection workflow.

Dimensions

110mm x 140mm

Material

Nylon

Sterility

Nonsterile

Wettability

Hydrophilic

• UV Crosslink Immobilization - Permanently fixes transferred nucleic acids for long-term storage and repeated high-sensitivity reprobing.
• Colony and Plaque Lifts - Captures nucleic acids at high density for hybridization screening of genomic and cDNA libraries.
• Northern Blotting - Anchors size-fractionated RNA at very high capacity for low-abundance transcript expression analysis.
• Low-Copy Detection - Captures scarce nucleic-acid targets at very high density for sensitive Southern and Northern hybridization.
• Multiple Reprobing - Withstands repeated stripping and reprobing across many high-sensitivity hybridization cycles per membrane.
• Southern Blotting - Immobilizes restriction-digested DNA on a supercharged surface for sensitive hybridization-based gene detection.