TURBOBL REFl NY-SPC 110x140mm SKU: 10416306

Nytran Nylon Blotting Membranes - 10416306

Southern and Northern blotting workflows produce the most reliable data when built on a positively charged nylon surface engineered for permanent nucleic acid immobilization under alkaline transfer conditions. Dual-cast manufacturing deposits nylon uniformly on both sides of an integrated support matrix, creating a symmetrical membrane that lies flat during transfer without the curling that disrupts capillary blotting setups. Optimized surface chemistry ensures efficient biomolecule capture and clear signal generation for publication-quality blotting results.

Membrane Specifications

• Manufactured with precise control over charge density and pore distribution, Nytran nylon membranes deliver consistent hybridization signal intensity across the entire membrane surface for reproducible blot-to-blot results.
• Chemical resistance to standard hybridization buffers, SSC solutions, and stripping reagents ensures reliable membrane performance across diverse blotting protocols.
• Alkaline transfer compatibility enables permanent nucleic acid immobilization without post-transfer UV crosslinking for streamlined Southern and Northern blot workflows.
• Very high positive surface charge creates strong ionic bonds with nucleic acid backbones for permanent target immobilization without post-transfer UV crosslinking.
• Nytran membranes combine high nucleic acid binding capacity with mechanical durability that withstands multiple rounds of stripping and reprobing, making them the preferred substrate for multi-probe hybridization experiments.

For your applications where target retention and background noise directly determine whether low-abundance bands are detectable, the surface chemistry and pore structure of this membrane give you the sensitivity margin to resolve what matters. Permanent immobilization of DNA and RNA under alkaline transfer conditions eliminates the UV crosslinking step, streamlining capillary blotting workflows while ensuring stable target retention through repeated probings. If your laboratory requires volume pricing, custom packaging configurations, or scheduled delivery programs, the Tisch Scientific sales team can structure a supply arrangement matched to your consumption rate.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Dimensions

110mm x 140mm

Material

Nylon

Sterility

Nonsterile

Wettability

Hydrophilic

• Nucleic Acid Library Screening — High-throughput identification of target clones from genomic and cDNA libraries using labeled probes.
• Restriction Fragment Length Analysis — Detection of polymorphic DNA fragments by Southern hybridization for genotyping and linkage mapping studies.
• Dot and Slot Blotting — Rapid nucleic acid quantitation and sequence-specific screening by direct sample application to charged nylon.
• Northern Blotting — Transfer and probe hybridization of total or mRNA for tissue-specific gene expression profiling and transcript characterization.
• Multiple Probe Rehybridization — Sequential stripping and reprobing of a single membrane with different labeled probes for multi-target analysis.
• Colony and Plaque Lifts — High-density library screening via in situ transfer and hybridization of bacterial colonies or bacteriophage plaques.