TURBOBL REFI NY-SPC 150x150mm
Whatman SKU: 10416314

Nylon Blotting Membranes, 0.45 µm, 15 cm x 15 cm, 1/pk - 10416314

Whatman's expertise in membrane casting guides the surface chemistry and quality control behind reproducible capture of transferred molecules. Densely charged nylon immobilizes low-copy-number nucleic acids after gel transfer. The high-density membrane carries one blot through many hybridization cycles and permanently fixes nucleic acids by UV crosslinking or baking. When low-copy-number targets must be seen, supercharged nylon captures nucleic acids at very high density for sensitive hybridization.

Key Characteristics

• The 0.45 micron pore suits transfer of larger DNA and RNA fragments onto the charged membrane
• Supercharged positive surface anchors nucleic acids at very high density
• Durable construction withstands repeated stripping and reprobing cycles
• Compatible with alkaline transfer and isotopic or non-isotopic detection
• UV crosslinking or baking permanently immobilizes nucleic acids on the membrane

Reliable nucleic-acid capture across the membrane keeps your hybridization signal clean and repeatable. Dependable nucleic-acid immobilization holds your transferred DNA and RNA through hybridization through baking or UV crosslinking for permanent fixation. Request a quality certificate, lot documentation, or a blanket-order quote from the Tisch Scientific support team.

Dimensions

150mm x 150mm

Material

Nylon

Sterility

Nonsterile

Wettability

Hydrophilic

• Colony and Plaque Lifts - Captures nucleic acids at high density for hybridization screening of genomic and cDNA libraries.
• Northern Blotting - Anchors size-fractionated RNA at very high capacity for low-abundance transcript expression analysis.
• Southern Blotting - Immobilizes restriction-digested DNA on a supercharged surface for sensitive hybridization-based gene detection.
• Low-Copy Detection - Captures scarce nucleic-acid targets at very high density for sensitive Southern and Northern hybridization.
• Multiple Reprobing - Withstands repeated stripping and reprobing across many high-sensitivity hybridization cycles per membrane.
• UV Crosslink Immobilization - Permanently fixes transferred nucleic acids for long-term storage and repeated high-sensitivity reprobing.