TURBOBL REFI NY-SPC 200x250mm SKU: 10416326

Nytran Nylon Blotting Membranes - 10416326

High-throughput genomic screening and gene expression analysis depend on nylon membranes that deliver consistent binding performance across every region of the membrane surface. Broad chemical compatibility with standard hybridization buffers, SSC and alkaline transfer solutions, and common stripping reagents ensures reliable performance across diverse blotting protocols. Quality-controlled manufacturing ensures the membrane consistency that demanding blotting applications require for reproducible, defensible results.

Membrane Specifications

• Engineered for Southern, Northern, colony lift, and dot blot applications, Nytran membranes support both radioactive and nonradioactive detection systems with the sensitivity needed for low-copy-number target sequences.
• Very uniform pore size and pore distribution across the membrane surface ensures consistent hybridization signal intensity for reproducible blot-to-blot comparisons.
• Chemical resistance to standard hybridization buffers, SSC solutions, and stripping reagents ensures reliable membrane performance across diverse blotting protocols.
• Rated at greater than 600 μg/cm² binding capacity, very high charge nylon construction captures target sequences with exceptional efficiency from complex genomic samples.
• Very high positive surface charge creates strong ionic bonds with nucleic acid backbones for permanent target immobilization without post-transfer UV crosslinking.

If your laboratory runs standardized blotting protocols across multiple analysts or shifts, the dimensional precision and binding uniformity of this membrane reduce the operator-to-operator variability that can compromise your data quality. Very high nucleic acid binding capacity combined with mechanical durability makes Nytran nylon membranes a reliable substrate for Southern, Northern, and colony blot applications requiring multiple hybridization rounds. If you need help matching a membrane chemistry and pore size to your sample matrix, Tisch Scientific's technical staff can walk you through the selection process and provide application-specific recommendations.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Dimensions

200mm x 250mm

Material

Nylon

Sterility

Nonsterile

Wettability

Hydrophilic

• Northern Blotting — Transfer and probe hybridization of total or mRNA for tissue-specific gene expression profiling and transcript characterization.
• Southern Blotting — Alkaline capillary transfer and hybridization of genomic DNA restriction fragments for gene detection and structural analysis.
• Nucleic Acid Library Screening — High-throughput identification of target clones from genomic and cDNA libraries using labeled probes.
• Restriction Fragment Length Analysis — Detection of polymorphic DNA fragments by Southern hybridization for genotyping and linkage mapping studies.
• Colony and Plaque Lifts — High-density library screening via in situ transfer and hybridization of bacterial colonies or bacteriophage plaques.
• Multiple Probe Rehybridization — Sequential stripping and reprobing of a single membrane with different labeled probes for multi-target analysis.