TURBOBL REFI NY-SPC 200x250mm
Whatman SKU: 10416326

Nylon Blotting Membranes, 0.45 µm, 20 cm x 25 cm, 1/pk - 10416326

Whatman holds surface and binding characteristics steady from lot to lot, giving laboratories the reproducibility that repeated transfer and detection work demands. High-binding supercharged nylon holds DNA and RNA through repeated hybridization rounds. The clean supercharged surface resolves low-copy-number nucleic-acid targets while carrying one blot through many hybridization cycles and locks DNA and RNA in place for repeated probing. For permanent immobilization at maximal capacity, UV-crosslinked supercharged nylon fixes DNA and RNA for repeated hybridization.

Key Characteristics

• Supercharged positive surface anchors nucleic acids at very high density
• Compatible with alkaline transfer and isotopic or non-isotopic detection
• The 0.45 micron pore suits transfer of larger DNA and RNA fragments onto the charged membrane
• Very high nucleic-acid binding above 600 ug/cm2 captures transferred DNA and RNA
• Extremely low background enables high-sensitivity low-copy-number detection

Holding transferred DNA or RNA firmly through hybridization keeps your Southern and Northern blots sensitive. Dependable nucleic-acid immobilization holds your transferred DNA and RNA through hybridization across capillary and alkaline transfer. Reach out to the Tisch Scientific team for volume pricing and technical support on your transfer membranes.

Dimensions

200mm x 250mm

Material

Nylon

Sterility

Nonsterile

Wettability

Hydrophilic

• Northern Blotting - Anchors size-fractionated RNA at very high capacity for low-abundance transcript expression analysis.
• Southern Blotting - Immobilizes restriction-digested DNA on a supercharged surface for sensitive hybridization-based gene detection.
• Multiple Reprobing - Withstands repeated stripping and reprobing across many high-sensitivity hybridization cycles per membrane.
• UV Crosslink Immobilization - Permanently fixes transferred nucleic acids for long-term storage and repeated high-sensitivity reprobing.
• Colony and Plaque Lifts - Captures nucleic acids at high density for hybridization screening of genomic and cDNA libraries.
• Low-Copy Detection - Captures scarce nucleic-acid targets at very high density for sensitive Southern and Northern hybridization.