Polyamide Membrane Circle (NL16), 0.2um Pore Size, 47mm, 100/Pk SKU: 10414012

Nylon Membrane Filters - 10414012

Designed to deliver reproducibility and confidence across critical sample types, the Whatman 10414012 provides the filtration consistency that method validation demands. Central to its design, verified 0.2 pore distribution across the Hydrophilic membrane surface guarantees batch-to-batch consistency in particle capture and analyte passage. For this reason, each membrane disc reflects a manufacturing standard where retention characteristics, physical integrity, and dimensional accuracy are verified before release.

Material Specifications

• Economical bulk packaging supports laboratories with high consumable throughput where individual sterile wrapping is unnecessary.
• High mechanical strength allows Nylon membranes to withstand elevated differential pressures without structural deformation.
• Broad compatibility with alcohols, aliphatic hydrocarbons, and aromatic solvents extends Nylon filtration into mixed-solvent sample preparation.
• Ideal for filtering aqueous biological solutions and mobile phases.
• Naturally hydrophilic and constructed with high tensile strength, Nylon provides reliable performance without pre-wetting in aqueous applications.

If your protocol requires direct on-membrane analysis by microscopy or culture, the surface characteristics and dimensional tolerances described above give you a substrate that performs consistently from disc to disc. In practice, nylon's compatibility with both aqueous and moderate organic solvent systems lets you standardize on one membrane type across HPLC sample prep workflows that alternate between reversed-phase and normal-phase mobile phases. Make the decision for consistency and reliability, ensuring that your laboratory maintains its reputation for high-quality, error-free output.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

50-65psi

Color

white

Diameter

47mm

Extractables

low

Filtration Area

17.35cm²

Flow Rate

20ml (Water)

Grid (Yes/No)

No

Material

Nylon

Maximum Operating Pressure

Holder-dependent

Maximum Operating Temperature (°C)

135°C

Membrane Thickness (µm)

110um

Pack Count

100

pH Range

3-14

Pore Size (µm)

0.2

Sterility

Nonsterile

Sterilization

Autoclavable at 121°C for 30 mins

Support Material

None

Wettability

Hydrophilic

• Radiolabeled Assays - Retains radiolabeled DNA/RNA fragments efficiently, ensuring accurate quantification in molecular biology experiments.
• Northern Blotting - Excellent for RNA transfer applications where high tensile strength is required for multiple rounds of probe stripping.
• Atmospheric Testing - Used in dry deposition sampling to capture specific atmospheric nitrates and sulfates for environmental quality studies.
• Solvent Mobilization - Clarifies organic mobile phases in glass vacuum holders, preventing particulate damage to sensitive HPLC pump seals.
• Gene Mapping Support - Provides a stable, charge-modified surface for DNA immobilization, facilitating precise gene mapping and analysis steps.
• Southern Blot Transfer - Binds nucleic acids strongly during capillary transfer, providing a durable matrix for repeated hybridization cycles.