Polyamide Membrane Circle (NL17), 0.45um Pore Size, 25mm, 100/Pk SKU: 10414106

Nylon Membrane Filters - 10414106

The Whatman 10414106 protects sensitive instrumentation and sample integrity by providing reliable particulate removal with minimal analyte adsorption or background interference. At its core, verified 0.45 pore distribution across the Hydrophilic membrane surface guarantees batch-to-batch consistency in particle capture and analyte passage. As designed, controlled production conditions produce membranes with documented pore size distribution and thickness tolerances, supporting method reproducibility across multiple filter lots.

Material Specifications

• Alkaline-resistant and compatible with a broad range of aqueous buffers used in pharmaceutical and biotechnology laboratories.
• Broad compatibility with alcohols, aliphatic hydrocarbons, and aromatic solvents extends Nylon filtration into mixed-solvent sample preparation.
• Supplied in bulk non-sterile packaging, offering an economical option for routine clarification and general laboratory filtration tasks.
• High mechanical strength allows Nylon membranes to withstand elevated differential pressures without structural deformation.
• Ideal for filtering aqueous biological solutions and mobile phases.

Maintaining accreditation under ISO 17025 or similar quality frameworks requires documented traceability at every step — the specification compliance of each membrane lot supports your ongoing audit readiness. With this in mind, alkaline resistance that other membranes cannot match means you can filter high-pH buffers, basic mobile phases, and caustic wash solutions without the membrane degradation that limits cellulose-based and PES alternatives. Elevate the quality of your analytical data today by standardizing on a filtration product built to meet the exact specifications of your applications.

For more information about chemical compatibility, please review our Chemical Compatibility Chart.

Bubble Point (psi)

32-46psi

Color

white

Diameter

25mm

Extractables

low

Filtration Area

4.91cm²

Flow Rate

> 50ml (Water)

Grid (Yes/No)

No

Material

Nylon

Maximum Operating Pressure

Holder-dependent

Maximum Operating Temperature (°C)

135°C

Membrane Thickness (µm)

110um

Pack Count

100

pH Range

3-14

Pore Size (µm)

0.45

Sterility

Nonsterile

Sterilization

Autoclavable at 121°C for 30 mins

Support Material

None

Wettability

Hydrophilic

• Northern Blotting - Excellent for RNA transfer applications where high tensile strength is required for multiple rounds of probe stripping.
• Southern Blot Transfer - Binds nucleic acids strongly during capillary transfer, providing a durable matrix for repeated hybridization cycles.
• Radiolabeled Assays - Retains radiolabeled DNA/RNA fragments efficiently, ensuring accurate quantification in molecular biology experiments.
• Atmospheric Testing - Used in dry deposition sampling to capture specific atmospheric nitrates and sulfates for environmental quality studies.
• Gene Mapping Support - Provides a stable, charge-modified surface for DNA immobilization, facilitating precise gene mapping and analysis steps.
• Alkaline Filtration - Withstands high-pH solutions during industrial fluid processing, offering durability where other polymers might degrade.