NC Hydrophilic Blotting Membrane Filter, 0.45um, 300mm x 4m, 1 Roll
Tisch SKU: BMNC453004

Nitrocellulose Blotting Membranes, 0.45 µm, 300 mm x 4 m, 1/roll - BMNC453004

Tisch Scientific works with established transfer-membrane manufacturers to ensure reliable quality and performance. Hydrophilic nitrocellulose binds transferred proteins for clean immunodetection. The nitrocellulose surface wets on contact with aqueous transfer buffer while sharpening signal in immunodetection and supports chemiluminescent, colorimetric, and fluorescent detection. When a Western blot needs clean, low-background signal, nitrocellulose holds the protein through detection.

Key Characteristics

• Optimized for aqueous electrophoretic transfer and immunodetection
• Low background sharpens signal across detection methods
• Compatible with chemiluminescent, colorimetric, fluorescent, and radioactive detection
• Well suited to low-molecular-weight proteins and lateral flow strips
• High protein binding captures transferred proteins for sensitive detection

Strong, even protein transfer and immobilization set up the sensitive detection your blots depend on. Dependable protein immobilization keeps your Western blot quantitative through your blocking, probing, and imaging steps. Send your target protein or nucleic acid details to our technical service team for a membrane recommendation.

Dimensions

300mm x 4m

Material

Nitrocellulose (NC)

Pack Count

1

Pore Size (µm)

0.45

Weight

2.06

Wettability

Hydrophilic

• Western Blotting - Captures transferred proteins for chemiluminescent, colorimetric, and fluorescent immunodetection workflows.
• Dot and Slot Blotting - Immobilizes directly applied protein samples for rapid semi-quantitative screening and detection assays.
• Lateral Flow Strips - Anchors capture proteins on nitrocellulose for point-of-care diagnostic test strip fabrication and assembly.
• Low-MW Protein Detection - Resolves low-molecular-weight proteins with sharp bands and clean, low-background detection signal.
• Immunoassay Development - Immobilizes antigens or antibodies on nitrocellulose for membrane-based immunoassay development and testing.
• Protein Immunodetection - Binds transferred proteins for antibody-based visualization across all common detection methods reliably.