Featuring a proprietary wash chemistry developed for FTA card workflows, this reagent purifies nucleic acids captured and stored on FTA cards so that DNA bound to the matrix is freed from contaminants prior to amplification. QIAcard FTA Wash Buffer removes PCR inhibitors and contaminants that can lower PCR yields, helping ensure high-quality DNA for STR analysis. The buffer is recommended for use with QIAcard FTA and Indicating FTA Card formats, as well as EasiCollect formats, and supplied in a 25 mL bottle.
Key Reagent Characteristics
- Designed to wash punches in the punch-in procedure for standard STR amplification, leaving DNA tightly bound to the FTA matrix.
- Removes PCR inhibitors and contaminants that can reduce amplification yields.
- Compatible with QIAcard FTA, Indicating FTA Card, and EasiCollect formats.
- Supports flexible sample scaling across various punch sizes and numbers.
- Promotes higher elution efficiency and reduced sample degradation.
Key Format Characteristics
- Supplied as a single 25 mL bottle.
- Intended for molecular biology applications, not for diagnosis, prevention, or treatment of disease.
- Used with cellulose-based FTA matrix workflows for room temperature collection, storage, and purification.
This wash buffer delivers consistent removal of inhibitors and contaminants, helping maintain sample integrity through downstream PCR. By keeping DNA bound to the matrix while washing away interfering substances, it supports reliable, reproducible amplification results.
- Capacity
- 25 mL
- Pack Count
- 1
- STR Analysis Washes FTA punches in the punch-in procedure to prepare clean template for standard short tandem repeat amplification.
- PCR Inhibitor Removal Removes inhibitors and contaminants from the FTA matrix that can lower PCR yields.
- Nucleic Acid Purification Purifies DNA captured and stored on QIAcard FTA, Indicating FTA, and EasiCollect card formats.
- Forensic Sample Prep Prepares archived blood, buccal, and other biological samples stored on FTA cards for downstream molecular analysis.
- DNA Archiving Workflows Supports room temperature collection and processing of nucleic acids from a wide variety of biological samples.
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