FluoroTrans W PVDF Transfer Membrane - 10x15cm Sheets, 10/Pk
Pall SKU: BSP0157

PVDF Blotting Membranes, 0.2 µm, 10 cm x 15 cm, 10/pk - BSP0157

Pall optimizes the FluoroTrans PVDF surface for sensitive capture of small peptides, holding immobilized bands firmly through repeated washing and probing steps. PVDF blotting membranes immobilize proteins after electrophoretic transfer. Hydrophobic PVDF activates with methanol before protein transfer while withstanding stripping and reprobing cycles and stays stable through harsh wash conditions. Where stripping and reprobing matter, mechanically strong PVDF carries the blot through repeated rounds.

Key Characteristics

• High protein binding captures and holds transferred proteins for sensitive detection
• Mechanically strong membrane withstands stripping and reprobing cycles
• Compatible with chemiluminescent, colorimetric, and fluorescent detection
• Hydrophobic surface activates with a brief methanol step before transfer
• Retains bound protein under SDS, urea, and organic-solvent wash conditions

Strong, even protein transfer and immobilization set up the sensitive detection your blots depend on. Dependable protein capture holds your transferred targets through detection across electrophoretic and semi-dry transfer. Ask our friendly customer service team to confirm the format and binding capacity your blotting protocol needs.

Dimensions

10cm x 15cm

Material

Polyvinylidene Fluoride (PVDF)

Sterility

Nonsterile

Wettability

Hydrophobic

• Quantitative Western - Supports reproducible, high-capacity protein capture for quantitative fluorescent Western workflows.
• Western Blotting - Captures electrophoretically transferred proteins for chemiluminescent and fluorescent immunodetection.
• Phosphoprotein Detection - Retains bound protein under stringent washes for phospho-specific antibody immunodetection assays.
• High-MW Protein Transfer - Holds high-molecular-weight proteins through transfer and detection with high binding capacity.
• Protein Sequencing - Immobilizes proteins on a chemically stable membrane for N-terminal sequencing and downstream analysis.
• Stripping and Reprobing - Withstands repeated stripping and reprobing cycles for multi-target analysis on a single membrane.