FluoroTrans W PVDF Transfer Membrane - 20x20cm Sheets, 10/Pk
Pall SKU: BSP0159

PVDF Blotting Membranes, 0.2 µm, 20 cm x 20 cm, 10/pk - BSP0159

Pall brings decades of membrane manufacturing expertise to Western transfer, producing a white microporous support with high protein-binding capacity. High-binding PVDF holds transferred proteins through stripping and reprobing. Hydrophobic PVDF activates with methanol before protein transfer while holding bound proteins firmly through detection and holds protein through stringent washing. For high-molecular-weight proteins and sequencing, PVDF immobilizes the target for reliable detection.

Key Characteristics

• Well suited to high-molecular-weight proteins and protein sequencing
• High protein binding captures and holds transferred proteins for sensitive detection
• Retains bound protein under SDS, urea, and organic-solvent wash conditions
• Mechanically strong membrane withstands stripping and reprobing cycles
• Compatible with chemiluminescent, colorimetric, and fluorescent detection

Capturing every transferred protein and holding each through detection keeps your Western results clean. Dependable protein capture holds your transferred targets through detection across electrophoretic and semi-dry transfer. Contact Tisch Scientific for help selecting the right blotting membrane for your transfer and detection workflow.

Dimensions

20cm x 20cm

Material

Polyvinylidene Fluoride (PVDF)

Sterility

Nonsterile

Wettability

Hydrophobic

• Protein Sequencing - Immobilizes proteins on a chemically stable membrane for N-terminal sequencing and downstream analysis.
• Phosphoprotein Detection - Retains bound protein under stringent washes for phospho-specific antibody immunodetection assays.
• High-MW Protein Transfer - Holds high-molecular-weight proteins through transfer and detection with high binding capacity.
• Quantitative Western - Supports reproducible, high-capacity protein capture for quantitative fluorescent Western workflows.
• Western Blotting - Captures electrophoretically transferred proteins for chemiluminescent and fluorescent immunodetection.
• Stripping and Reprobing - Withstands repeated stripping and reprobing cycles for multi-target analysis on a single membrane.