PVDF Blotting Membranes, 0.2 µm, 26 cm x 3.3 m, 1/roll - BSP0161
Pall's FluoroTrans PVDF membrane supports chemiluminescent, fluorescent, and colorimetric detection, presenting captured proteins against a very low background. PVDF blotting membranes immobilize proteins after electrophoretic transfer. Chemically stable PVDF holds protein through stringent washing while holding up to repeated handling and readies for electrophoretic transfer. When a Western blot demands durable, high-capacity capture, the PVDF membrane holds the protein through detection.
Key Characteristics
- Retains bound protein under SDS, urea, and organic-solvent wash conditions
- High protein binding captures and holds transferred proteins for sensitive detection
- Compatible with chemiluminescent, colorimetric, and fluorescent detection
- Well suited to high-molecular-weight proteins and protein sequencing
- Hydrophobic surface activates with a brief methanol step before transfer
Strong, even protein transfer and immobilization set up the sensitive detection your blots depend on. Dependable protein immobilization keeps your Western blot quantitative so low-abundance targets stay visible. Reach out to the Tisch Scientific team for volume pricing and technical support on your transfer membranes.
- Dimensions
- 26cm x 3.3m
- Material
- Polyvinylidene Fluoride (PVDF)
- Sterility
- Nonsterile
- Wettability
- Hydrophobic
- Protein Sequencing - Immobilizes proteins on a chemically stable membrane for N-terminal sequencing and downstream analysis.
- Western Blotting - Captures electrophoretically transferred proteins for chemiluminescent and fluorescent immunodetection.
- Phosphoprotein Detection - Retains bound protein under stringent washes for phospho-specific antibody immunodetection assays.
- Stripping and Reprobing - Withstands repeated stripping and reprobing cycles for multi-target analysis on a single membrane.
- Quantitative Western - Supports reproducible, high-capacity protein capture for quantitative fluorescent Western workflows.
- High-MW Protein Transfer - Holds high-molecular-weight proteins through transfer and detection with high binding capacity.