PES Centrifugal Filters, 100kDa MWCO, 50mL Conical, White Cap, Nonsterile, 96/Pk
GVS SKU: CEF50W100KD24040

Featuring a hydrophilic polyethersulfone (PES) ultrafiltration membrane housed in a 50mL conical tube format, these centrifugal filter units deliver fast, gentle concentration and purification of biological samples by molecular size. The 100kDa molecular weight cut-off retains macromolecules above the threshold while allowing smaller solutes and solvent to pass into the collection tube, supporting protein concentration, buffer exchange, and desalting workflows. Each unit provides an active membrane area of 5.48 cm² and operates across a temperature range of 0 to 40 degrees C.

Key PES Membrane Characteristics

• Hydrophilic polyethersulfone (PES) membrane separates molecules primarily by size for reliable ultrafiltration.
• 100kDa molecular weight cut-off retains macromolecules above the threshold while passing smaller solutes.
• Active membrane area of 5.48 cm² supports efficient sample processing.
• Vertical membrane design promotes fast filtration and high recovery of dilute samples.
• Suited to concentration, purification, buffer exchange, and desalting of proteins and other biomolecules.

Key Construction Characteristics

• Polypropylene (PP) 50mL conical centrifuge tube fits centrifuges that accept standard 50mL conical tubes.
• White polyethylene (PE) cap with sealing gasket; filter tube constructed of MBS with a PE filter slice.
• Maximum centrifugal force of 3,000 x g in a swinging bucket rotor and 4,000 x g in a fixed angle rotor.
• Supplied nonsterile, 24 pieces per box, 4 boxes per case (96 units total).
• Final concentrate volume of 200 microliters with a maximum sample volume of 15mL (swinging bucket) or 12mL (fixed angle rotor).

These units deliver consistent, reproducible separation that helps maintain the integrity of labile and poorly expressed proteins. The simple spin-and-recover format minimizes transfer steps and sample handling, supporting clean concentrate recovery and meaningful downstream data.

Capacity

50 mL

Color

White

Construction

PP conical tube, PE cap/gasket, MBS filter tube, PE filter slice, PES membrane

Filtration Area

5.48 cm²

Holdup Volume

200 µL final concentrate volume

Material

Polyethersulfone (PES)

Maximum Operating Temperature (°C)

40

Membrane Brand

GVS

MWCO(Da)

100000

Pack Count

96

Sterility

Nonsterile

Wettability

Hydrophilic

• Protein Concentration Concentrates dilute protein solutions above 100kDa by removing water and smaller solutes through the PES membrane.
• Buffer Exchange Facilitates exchange of sample buffers by repeated dilution and centrifugal filtration of retained macromolecules.
• Desalting Removes salts and low molecular weight contaminants while retaining target macromolecules above the cut-off.
• Sample Purification Purifies biological samples by separating high molecular weight species from smaller components.
• Nucleic Acid Concentration Concentrates and recovers nucleic acids and other biomolecules exceeding the 100kDa threshold.
• Diafiltration Prep Prepares concentrated retentate for downstream analysis with minimal transfer steps and sample loss.