Featuring a naturally hydrophilic polyethersulfone (PES) ultrafiltration membrane housed in a 50mL conical tube format, this centrifugal filter delivers fast, gentle concentration and purification of biological samples by molecular size. With a 10kDa molecular weight cut-off (MWCO) and a 5.48 cm² active membrane area, the device retains macromolecules above the cut-off while passing smaller solutes, buffers, and salts into the filtrate. It fits centrifuges that accept standard 50mL conical tubes and operates across a temperature range of 0°C to 40°C.
Key PES Membrane Characteristics
- Naturally hydrophilic polyethersulfone (PES) membrane for efficient wetting and consistent flow
- 10kDa molecular weight cut-off (MWCO) retains macromolecules while passing smaller solutes
- Active membrane area of 5.48 cm² supports rapid sample processing
- Separation based on molecular size, suited to concentration, desalting, and buffer exchange
- Designed for gentle handling of labile and poorly expressed proteins
Key Construction Characteristics
- Filter material of Acrylonitrile-Butadiene-Styrene (ABS) with a polypropylene centrifuge tube
- White polyethylene cap and sealing gasket
- Maximum sample volume of 15mL (swinging bucket rotor) and 12mL (fixed angle rotor)
- Maximum centrifugal force of 3,000 x g (swinging bucket) and 4,000 x g (fixed angle)
- Nonsterile; supplied 24 pieces per box, 4 boxes per case
This centrifugal concentrator maintains sample integrity through a simple, one-step procedure that minimizes transfer steps and handling. The hydrophilic PES membrane provides reliable, reproducible recovery of concentrated retentate, making it well suited to protein and nucleic acid work where biologically active yields matter.
- Capacity
- 50 mL conical tube
- Color
- White
- Construction
- ABS (Acrylonitrile-Butadiene-Styrene) filter tube, polypropylene centrifuge tube, polyethylene cap and sealing gasket
- Filtration Area
- 5.48 cm²
- Material
- Polyethersulfone (PES)
- Maximum Operating Temperature (°C)
- 40
- Membrane Color
- White
- MWCO(Da)
- 10000
- Pack Count
- 24/Box, 4 Boxes/Case (96 total)
- Sterility
- Nonsterile
- Volume Throughput (ml)
- Maximum sample volume: 15 mL (swinging bucket), 12 mL (fixed angle rotor); Final concentrate volume: 200 µL
- Wettability
- Hydrophilic
- Protein Concentration Concentrates dilute protein solutions by retaining species above the 10kDa cut-off while passing buffer and water into the filtrate.
- Buffer Exchange and Desalting Removes low-molecular-weight salts and buffer components to prepare samples for downstream analysis.
- Nucleic Acid Concentration Concentrates and recovers nucleic acid samples using size-based ultrafiltration.
- Sample Purification Separates target macromolecules from smaller contaminants for cleaner preparations.
- Recovery of Labile Proteins Provides gentle, one-step processing that protects labile and poorly expressed proteins from variability introduced by multiple transfer steps.
- Concentration of Biological Samples Handles biological samples up to 15mL in swinging bucket rotors and 12mL in fixed angle rotors.