Featuring a low-binding hydrophilic polyethersulfone (PES) ultrafiltration membrane paired with a standard 50mL conical tube, this centrifugal filter device concentrates and purifies biological samples by molecular weight. The membrane carries a 30kDa molecular weight cut-off (MWCO), retaining macromolecules above the cut-off in the retentate while smaller solutes and water pass into the filtrate. A vertical membrane orientation promotes fast sample processing and high recovery while maintaining a gentle environment for labile and poorly expressed proteins.
Key PES Membrane Characteristics
- Hydrophilic polyethersulfone (PES) membrane with a defined 30kDa molecular weight cut-off for ultrafiltration of biological samples.
- Low protein and nucleic acid binding supports high sample recovery.
- Retains molecules above the MWCO while passing water and smaller solutes into the filtrate.
- Vertical membrane design provides fast sample processing and helps minimize fouling.
- Suited to concentration, desalting, and buffer exchange of proteins, nucleic acids, and similar biomolecules.
Key Construction Characteristics
- Housing constructed from acrylonitrile butadiene styrene (ABS) with a polypropylene (PP) 50mL conical centrifuge tube.
- White cap and sealing gasket made of polyethylene (PE).
- Fits centrifuges that accept standard 50mL conical tubes.
- Supplied nonsterile, 8 pieces per box, 12 boxes per case (96 per case).
- Operating temperature range of 0 degrees C to 40 degrees C.
This device delivers reliable, repeatable concentration of dilute samples while preserving target molecule integrity. The dead-stop feature helps prevent samples from spinning to dryness, protecting valuable sample volumes. The combination of a low-binding PES membrane and a standard conical format provides a straightforward, gentle workflow for downstream analysis.
- Capacity
- 50mL conical tube
- Color
- White
- Material
- Polyethersulfone (PES)
- Maximum Operating Temperature (°C)
- 40
- MWCO(Da)
- 30000
- Pack Count
- 96
- Sterility
- Nonsterile
- Wettability
- Hydrophilic
- Protein Concentration Concentrates dilute protein solutions by retaining species above the 30kDa cut-off while removing buffer and smaller solutes.
- Buffer Exchange Facilitates exchange of sample buffers during preparation of biological samples for downstream work.
- Desalting Removes salts and low molecular weight contaminants from macromolecule samples.
- Nucleic Acid Sample Prep Concentrates and purifies nucleic acid samples using the low-binding PES membrane.
- Biological Sample Purification Separates sample components by molecular weight for clarification and purification of biological samples.
- Viral Particle Concentration Concentrates virus particles and other macromolecules above the molecular weight cut-off.