Featuring a hydrophobic PTFE membrane sealed within an all-in-one sample preparation device, this Separa syringeless filter vial integrates a self-sampling system, filtration membrane, auto-sampler vial, and a precut septa cap into a single unit. The 0.2um PTFE membrane delivers superior chemical resistance for strong acids and aggressive organic solvents, while the single-step plunger design filters and presents the sample ready for autosampler loading. Liquid is placed in the outer vial and dispensed by pressing the inner insert, pushing the sample through the membrane. The device provides a chamber volume of 480ul, a filter capacity of 450ul, and a dead volume as low as 30ul, with a compression force of 0.6 bar (8 psi).
Key PTFE Membrane Characteristics
- Hydrophobic, chemically and biologically inert PTFE membrane with superior chemical resistance
- Compatible with strong acids and aggressive aqueous and organic solvent solutions
- 0.2um pore size for clarification and particulate removal prior to chromatographic analysis
- Sealed within an internal insert membrane chamber for clean, contained filtration
- Belongs to a PFAS-free verified Separa product line
Key Construction Characteristics
- Housing constructed of polypropylene with a silicone septa, measuring 12mm diameter by 32mm height
- Precut (pre-slit) septa cap ensures easy and clean sample transfer
- Maximum operating temperature of 50C (120F)
- Nonsterile, supplied 100 per pack in a pink cap color
- Compatible with most standard HPLC and uHPLC autosamplers and multi-compressors
This syringeless format replaces the separate syringe, syringe filter, glass vial, and cap, reducing waste and consolidating multiple steps into one. The integrated design maintains sample integrity by minimizing transfer steps and the associated risk of cross-contamination. After a single press, the filtered sample is immediately ready for reliable analysis.
- Capacity
- Filter capacity 450 µl; chamber volume 480 µl
- Color
- Pink
- Compliance Standards
- PFAS-Free Verified
- Construction
- Polypropylene housing with PTFE membrane and silicone septa
- Diameter
- 12 mm
- Dimensions
- 12 mm dia. x 32 mm H
- Holdup Volume
- 30 µl (dead volume)
- Length
- 32 mm
- Material
- Polytetrafluoroethylene (PTFE)
- Maximum Operating Temperature (°C)
- 50
- Pack Count
- 100
- Pore Size (µm)
- 0.2
- Septa Material
- Silicone
- Sterility
- Nonsterile
- Vial Cap Type
- Precut (pre-slit) septa cap
- Wettability
- Hydrophobic
- HPLC and uHPLC Sample Preparation Prepares clarified, particulate-free samples in a single step ready for direct loading into standard autosamplers.
- Clarification of Aggressive Solvents The hydrophobic, chemically inert PTFE membrane withstands strong acids and aggressive organic solvent solutions.
- Drug Metabolite Studies (Protein Precipitation) Clarifies protein-precipitated samples for downstream chromatographic and analytical workflows.
- Chromatography Sample Prep Removes particulates from aqueous and organic samples to protect columns and improve analytical results.
- Aqueous and Organic Sample Clarification Provides reliable clarification of both aqueous and organic solvent solutions prior to analysis.
- Automated Sample Processing Compatible with most standard autosamplers and multi-compressors for higher-throughput sample preparation.