PVDF Hydrophobic Blotting Membrane Filter, 0.22um, 300mm x 3m, 1 Roll
Tisch SKU: BMPVDF223003

PVDF Blotting Membranes, 0.22 µm, 300 mm x 3 m, 1/roll - BMPVDF223003

Tisch Scientific customer service specialists focus on laboratory products and can help source the right solution for specific applications. PVDF blotting membranes immobilize proteins after electrophoretic transfer. PVDF supports chemiluminescent, colorimetric, and fluorescent detection while holding bound proteins firmly through detection and stays stable through harsh wash conditions. When a Western blot demands durable, high-capacity capture, the PVDF membrane holds the protein through detection.

Key Characteristics

• High protein binding captures and holds transferred proteins for sensitive detection
• Compatible with chemiluminescent, colorimetric, and fluorescent detection
• Mechanically strong membrane withstands stripping and reprobing cycles
• Hydrophobic surface activates with a brief methanol step before transfer
• Retains bound protein under SDS, urea, and organic-solvent wash conditions

Reliable protein capture across the membrane keeps your immunodetection sharp from transfer to signal. Dependable protein immobilization keeps your Western blot quantitative so low-abundance targets stay visible. Ask the Tisch Scientific team about precut sheets, rolls, and bulk pricing for your blotting workflows.

Dimensions

300mm x 3m

Material

Polyvinylidene Fluoride (PVDF)

Pack Count

1

Pore Size (µm)

0.22

Weight

2.06

Wettability

Hydrophobic

• Western Blotting - Captures electrophoretically transferred proteins for chemiluminescent and fluorescent immunodetection.
• Protein Sequencing - Immobilizes proteins on a chemically stable membrane for N-terminal sequencing and downstream analysis.
• Stripping and Reprobing - Withstands repeated stripping and reprobing cycles for multi-target analysis on a single membrane.
• Phosphoprotein Detection - Retains bound protein under stringent washes for phospho-specific antibody immunodetection assays.
• High-MW Protein Transfer - Holds high-molecular-weight proteins through transfer and detection with high binding capacity.
• Quantitative Western - Supports reproducible, high-capacity protein capture for quantitative fluorescent Western workflows.