PVDF Hydrophobic Blotting Membrane Filter, 0.22um, 300mm x 4m, 1 Roll
Tisch SKU: BMPVDF223004

PVDF Blotting Membranes, 0.22 µm, 300 mm x 4 m, 1/roll - BMPVDF223004

Tisch Scientific customer service specialists focus on laboratory products and can help source the right solution for specific applications. PVDF blotting membranes immobilize proteins after electrophoretic transfer. Hydrophobic PVDF activates with methanol before protein transfer and carries high-molecular-weight proteins through detection. For high-molecular-weight proteins and sequencing, PVDF immobilizes the target for reliable detection.

Key Characteristics

• Retains bound protein under SDS, urea, and organic-solvent wash conditions
• Mechanically strong membrane withstands stripping and reprobing cycles
• Well suited to high-molecular-weight proteins and protein sequencing
• Hydrophobic surface activates with a brief methanol step before transfer
• High protein binding captures and holds transferred proteins for sensitive detection

Capturing every transferred protein and holding each through detection keeps your Western results clean. Dependable protein capture holds your transferred targets through detection from blocking through antibody probing and imaging. Contact Tisch Scientific for help selecting the right blotting membrane for your transfer and detection workflow.

Dimensions

300mm x 4m

Material

Polyvinylidene Fluoride (PVDF)

Pack Count

1

Pore Size (µm)

0.22

Weight

2.06

Wettability

Hydrophobic

• Quantitative Western - Supports reproducible, high-capacity protein capture for quantitative fluorescent Western workflows.
• Phosphoprotein Detection - Retains bound protein under stringent washes for phospho-specific antibody immunodetection assays.
• Protein Sequencing - Immobilizes proteins on a chemically stable membrane for N-terminal sequencing and downstream analysis.
• Western Blotting - Captures electrophoretically transferred proteins for chemiluminescent and fluorescent immunodetection.
• High-MW Protein Transfer - Holds high-molecular-weight proteins through transfer and detection with high binding capacity.
• Stripping and Reprobing - Withstands repeated stripping and reprobing cycles for multi-target analysis on a single membrane.