PVDF Hydrophobic Blotting Membrane Filter, 0.45um, 300mm x 3m, 1 Roll
Tisch SKU: BMPVDF453003

PVDF Blotting Membranes, 0.45 µm, 300 mm x 3 m, 1/roll - BMPVDF453003

Tisch Scientific carries a broad laboratory catalog covering transfer membranes and related consumables for the laboratory. PVDF blotting membranes immobilize proteins after electrophoretic transfer. Methanol-activated PVDF readies for electrophoretic transfer and suits Western detection and protein sequencing. When a Western blot demands durable, high-capacity capture, the PVDF membrane holds the protein through detection.

Key Characteristics

• Well suited to high-molecular-weight proteins and protein sequencing
• Hydrophobic surface activates with a brief methanol step before transfer
• Mechanically strong membrane withstands stripping and reprobing cycles
• Retains bound protein under SDS, urea, and organic-solvent wash conditions
• High protein binding captures and holds transferred proteins for sensitive detection

Strong, even protein transfer and immobilization set up the sensitive detection your blots depend on. Dependable protein immobilization keeps your Western blot quantitative across chemiluminescent, colorimetric, and fluorescent detection. Ask our friendly customer service team to confirm the format and binding capacity your blotting protocol needs.

Dimensions

300mm x 3m

Material

Polyvinylidene Fluoride (PVDF)

Pack Count

1

Pore Size (µm)

0.45

Weight

2.06

Wettability

Hydrophobic

• Stripping and Reprobing - Withstands repeated stripping and reprobing cycles for multi-target analysis on a single membrane.
• Quantitative Western - Supports reproducible, high-capacity protein capture for quantitative fluorescent Western workflows.
• Protein Sequencing - Immobilizes proteins on a chemically stable membrane for N-terminal sequencing and downstream analysis.
• Western Blotting - Captures electrophoretically transferred proteins for chemiluminescent and fluorescent immunodetection.
• High-MW Protein Transfer - Holds high-molecular-weight proteins through transfer and detection with high binding capacity.
• Phosphoprotein Detection - Retains bound protein under stringent washes for phospho-specific antibody immunodetection assays.