PVDF Hydrophobic Blotting Membrane Filter, 0.45um, 300mm x 4m, 1 Roll
Tisch SKU: BMPVDF453004

PVDF Blotting Membranes, 0.45 µm, 300 mm x 4 m, 1/roll - BMPVDF453004

Tisch Scientific carries a broad laboratory catalog covering transfer membranes and related consumables for the laboratory. High-binding PVDF holds transferred proteins through stripping and reprobing. The PVDF membrane binds transferred proteins at high capacity while retaining bound protein under SDS and urea and supports multiple reprobing rounds. Where stripping and reprobing matter, mechanically strong PVDF carries the blot through repeated rounds.

Key Characteristics

• Compatible with chemiluminescent, colorimetric, and fluorescent detection
• Retains bound protein under SDS, urea, and organic-solvent wash conditions
• Well suited to high-molecular-weight proteins and protein sequencing
• Mechanically strong membrane withstands stripping and reprobing cycles
• High protein binding captures and holds transferred proteins for sensitive detection

Reliable protein capture across the membrane keeps your immunodetection sharp from transfer to signal. Dependable protein immobilization keeps your Western blot quantitative so low-abundance targets stay visible. Connect with our customer support technicians to choose between PVDF, nitrocellulose, and nylon for your blots.

Dimensions

300mm x 4m

Material

Polyvinylidene Fluoride (PVDF)

Pack Count

1

Pore Size (µm)

0.45

Weight

2.06

Wettability

Hydrophobic

• Western Blotting - Captures electrophoretically transferred proteins for chemiluminescent and fluorescent immunodetection.
• Protein Sequencing - Immobilizes proteins on a chemically stable membrane for N-terminal sequencing and downstream analysis.
• Stripping and Reprobing - Withstands repeated stripping and reprobing cycles for multi-target analysis on a single membrane.
• Quantitative Western - Supports reproducible, high-capacity protein capture for quantitative fluorescent Western workflows.
• High-MW Protein Transfer - Holds high-molecular-weight proteins through transfer and detection with high binding capacity.
• Phosphoprotein Detection - Retains bound protein under stringent washes for phospho-specific antibody immunodetection assays.